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Anti-Cytochrome C 抗体 (ab18738)

Submit a review Q&A (1)References (11)
Western blot - Anti-Cytochrome C antibody (ab18738)
  • Western blot - Anti-Cytochrome C antibody (ab18738)

Key features and details

  • Rabbit polyclonal to Cytochrome C
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cytochrome C antibody
    Cytochrome C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cytochrome C

  • 由来種

    Rabbit

  • 特異性

    This antibody detects the 15 kDa cytochrome C.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken

  • 免疫原

    Synthetic peptide corresponding to Rat Cytochrome C. Corresponding to residues surrounding amino acid 70 of rat Cytochrome C.
    (Peptide available as ab38265)

  • ポジティブ･コントロール

    • WB: Jurkat cell lysate and 3T3 cell lysate. Rat kidney cell lysate.

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called 3025 Cytochrome c Antibody. 3025-100 is the same size as the 100 µg size of ab18738.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18738の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa.
特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa.

ターゲット情報

  • 機能

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

  • 関連疾患

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

  • 配列類似性

    Belongs to the cytochrome c family.

  • 翻訳後修飾

    Binds 1 heme group per subunit.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

画像

  • Western blot - Anti-Cytochrome C antibody (ab18738)
    Western blot - Anti-Cytochrome C antibody (ab18738)

    Western Blot analysis of Left Lane: Jurkat cell lysate and Right Lane: 3T3 cell lysates labeling Cytochrome C with ab18738.

  • Western blot - Anti-Cytochrome C antibody (ab18738)
    Western blot - Anti-Cytochrome C antibody (ab18738)
    Anti-Cytochrome C antibody (ab18738) + Rat kidney cell lysates

    Predicted band size: 12 kDa

参考文献 (11)

ab18738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18738 は 11 報の論文で使用されています。

  • E L  et al. MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1. Exp Ther Med 17:2152-2160 (2019). PubMed: 30783480
  • Liu H  et al. The ginsenoside Rk3 exerts anti-esophageal cancer activity in vitro and in vivo by mediating apoptosis and autophagy through regulation of the PI3K/Akt/mTOR pathway. PLoS One 14:e0216759 (2019). PubMed: 31091245
  • Kalpage HA  et al. Serine-47 phosphorylation of cytochrome c in the mammalian brain regulates cytochrome c oxidase and caspase-3 activity. FASEB J 33:13503-13514 (2019). PubMed: 31570002
  • Stancic A  et al. A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection. Comp Biochem Physiol B Biochem Mol Biol 219-220:1-9 (2018). WB . PubMed: 29501789
  • Dotiwala F  et al. Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program. Cell 171:1125-1137.e11 (2017). PubMed: 29107333
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Could you tell me the immunogen sequence or the homology between the immunogen and the chicken sequence? The region immediately around amino acid 70 is conserved, but the homology goes down further out from that residue.

Read More
Answer

Thank you for your enquiry. I'm happy to report that the immunogen shares 100% homology with the sequence in chicken. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

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