Anti-Cytochrome C 抗体 [7H8.2C12] (ab13575)
製品の概要
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製品名
Anti-Cytochrome C antibody [7H8.2C12]
Cytochrome C 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7H8.2C12] to Cytochrome C -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IHC-Fr, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Horse, Pigeon, Human, Drosophila melanogaster -
免疫原
corresponding to Cytochrome C.
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エピトープ
The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, based on competitive ELISA results. -
ポジティブ･コントロール
- WB: HeLa, Jurkat and human heart whole cell lysates; IHC-P: Human liver and skin tissues; ICC/IF: Leukocytes from murine bone marrow; FC: HepG2 cells.
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
7H8.2C12 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab13575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 0.1-1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/500. (see Abreviews) | |
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
関連疾患
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. -
配列類似性
Belongs to the cytochrome c family. -
翻訳後修飾
Binds 1 heme group per subunit. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 100053958 Horse
- Entrez Gene: 54205 Human
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P00004 Horse
- SwissProt: P99999 Human
- SwissProt: P62897 Mouse
see all -
別名
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
画像
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![Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20882-anti-cytochrome-c-antibody-7h82c12-western-blot.jpg)
Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)All lanes : Anti-Cytochrome C antibody [7H8.2C12] (ab13575) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Additional bands at: 14 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesAbcam recommends using milk (5%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20884-anti-cytochrome-c-antibody-7h82c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)IHC image of Cytochrome C staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13575, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20883-anti-cytochrome-c-antibody-7h82c12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)Image courtesy of an anonymous Abreview.ab13575 staining Cytochrome C in leukocytes from murine bone marrow by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with primary antibody at 1/250 for 16 hours at 5°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution. Counterstained with DAPI (blue). -
![Immunohistochemistry (Frozen sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-281386-anti-cytochrome-c-antibody-7h82c12-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)This image is courtesy of an anonymous Abreviewab13575 staining Cytochrome C from Rat liver by Immunohistochemistry (IHC-Fr-frozen sections).Tissue was fixed with acetone,Samples were incubated with primary antibody (1/300 in PBS 0.1% Triton X) for 15 hours at 4°C.Alexa Fluor® 555 Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20881-anti-cytochrome-c-antibody-7h82c12-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)Overlay histogram showing HepG2 cells stained with ab13575 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13575, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1]/mouse IgG2b [PLPV219] (ab91353/ab91366, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20886-anti-cytochrome-c-antibody-7h82c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)Ab13575 staining human normal skin tissue. Staining is localised to mitochondria.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
参考文献
This product has been referenced in:
- Gopisetty MK et al. Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells. J Nanobiotechnology 17:9 (2019). Read more (PubMed: 30670028) »
- Zhang H et al. Adenovirus-mediated herpes simplex virus thymidine kinase gene therapy combined with ganciclovir induces hepatoma cell apoptosis. Exp Ther Med 17:1649-1655 (2019). Read more (PubMed: 30783433) »
