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Anti-Cytochrome C 抗体 [7H8.2C12] (ab13575)

  • Datasheet
  • SDS
Reviews (31)Q&A (4)References (175)

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Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
  • Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)

Key features and details

  • Mouse monoclonal [7H8.2C12] to Cytochrome C
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

HRP

こちらの製品もご検討ください

タンパク質
Recombinant Human Cytochrome C protein (ab172817)
アッセイ
Product image
Cytochrome c Release Assay Kit (ab65311)

関連製品

製品の概要

  • 製品名

    Anti-Cytochrome C antibody [7H8.2C12]
    Cytochrome C 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [7H8.2C12] to Cytochrome C
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Pigeon, Drosophila melanogaster
  • 免疫原

    This information is proprietary to Abcam and/or its suppliers.

  • エピトープ

    The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, based on competitive ELISA results.
  • ポジティブ・コントロール

    • WB: HeLa, Jurkat and human heart whole cell lysates; IHC-P: Human liver and skin tissues; ICC/IF: Leukocytes from murine bone marrow; Flow Cyt: HepG2 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    7H8.2C12
  • アイソタイプ

    IgG2b
  • 軽鎖の種類

    kappa
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Cytochrome C
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Cytochrome C
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Alternative Versions

    • HRP Anti-Cytochrome C antibody [7H8.2C12] (ab199222)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Cytochrome C protein (ab172817)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13575の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 0.1-1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB (20)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa).
IHC-P (2)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt
Use 0.1-1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 関連疾患

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • 配列類似性

    Belongs to the cytochrome c family.
  • 翻訳後修飾

    Binds 1 heme group per subunit.
  • 細胞内局在

    Mitochondrion matrix.
  • Target information above from: UniProt accession P99999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 100053958 Horse
    • Entrez Gene: 54205 Human
    • Entrez Gene: 13063 Mouse
    • Entrez Gene: 25309 Rat
    • Omim: 123970 Human
    • SwissProt: P00004 Horse
    • SwissProt: P99999 Human
    • SwissProt: P62897 Mouse
    • SwissProt: P62898 Rat
    • Unigene: 437060 Human
    • Unigene: 617193 Human
    see all
  • 別名

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

画像

  • Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    All lanes : Anti-Cytochrome C antibody [7H8.2C12] (ab13575) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Additional bands at: 14 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Abcam recommends using milk (5%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    IHC image of Cytochrome C staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13575, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)

    Overlay histogram showing HepG2 cells stained with ab13575 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13575, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1]/mouse IgG2b [PLPV219] (ab91353/ab91366, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
    Ab13575 staining human normal skin tissue. Staining is localised to mitochondria.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (175)

ab13575 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13575 は 175 報の論文で使用されています。

  • Jia G  et al. Neural stem cell-conditioned medium ameliorates Aß25-35-induced damage in SH-SY5Y cells by protecting mitochondrial function. Bosn J Basic Med Sci 21:179-186 (2021). PubMed: 32156251
  • Jing L  et al. Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells. BMC Complement Med Ther 21:18 (2021). PubMed: 33413359
  • Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
  • Liu L  et al. The natural product lapiferin inhibits cell proliferation and promotes cell apoptosis in gingival squamous cell carcinoma via P21 regulation. Mol Med Rep 24:N/A (2021). PubMed: 33907850
  • Devenport SN  et al. Colorectal cancer cells utilize autophagy to maintain mitochondrial metabolism for cell proliferation under nutrient stress. JCI Insight 6:N/A (2021). PubMed: 34138755
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 35 Abreviews or Q&A

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Lucilia sericata Cell lysate - other (Sub-mitochondrial particles isolated from wing mus)
Gel Running Conditions
Reduced Denaturing (4-15% gel)
Loading amount
10 µg
Treatment
Solubilised with 2.5% SMA
Specification
Sub-mitochondrial particles isolated from wing mus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 May 12 2021

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Mar 23 2021

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 May 14 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [7H8.2C12]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (H9c2 rat cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
H9c2 rat cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 18 2020

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat gastronecimus muscle)
Gel Running Conditions
Reduced Denaturing (10% SDS - PAGE)
Loading amount
20 µg
Treatment
Durg
Specification
Rat gastronecimus muscle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 10 2019

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 cell)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
PC12 cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

MISS. Ji Yeon Lee

Verified customer

投稿 Jan 29 2019

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - other (cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (gel 12%)
Loading amount
60 µg
Specification
cardiomyocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 17 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lymph node dexamethasone treated rat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (Citrate)
Permeabilization
No
Specification
Lymph node dexamethasone treated rat
Fixative
Paraformaldehyde
Read More

Dr. Li Li

Verified customer

投稿 Jun 05 2017

Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (Cytosolic Fractions from Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Cytosolic Fractions from Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 18 2016

1-10 of 35 Abreviews or Q&A

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