Anti-Cytochrome C 抗体 [7H8.2C12] (ab13575)
![Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20882-anti-cytochrome-c-antibody-7h82c12-western-blot.jpg "Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)")
Key features and details
- Mouse monoclonal [7H8.2C12] to Cytochrome C
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytochrome C antibody [7H8.2C12]
Cytochrome C 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7H8.2C12] to Cytochrome C -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Pigeon, Drosophila melanogaster
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免疫原
This information is proprietary to Abcam and/or its suppliers.
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エピトープ
The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, based on competitive ELISA results. -
ポジティブ･コントロール
- WB: HeLa, Jurkat and human heart whole cell lysates; IHC-P: Human liver and skin tissues; ICC/IF: Leukocytes from murine bone marrow; Flow Cyt: HepG2 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
7H8.2C12 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13575の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.1-1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| WB | (20) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa).
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| IHC-P | (2) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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Flow Cyt
Use 0.1-1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
関連疾患
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. -
配列類似性
Belongs to the cytochrome c family. -
翻訳後修飾
Binds 1 heme group per subunit. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 100053958 Horse
- Entrez Gene: 54205 Human
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P00004 Horse
- SwissProt: P99999 Human
- SwissProt: P62897 Mouse
see all -
別名
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
画像
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Western blot - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)All lanes : Anti-Cytochrome C antibody [7H8.2C12] (ab13575) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Additional bands at: 14 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesAbcam recommends using milk (5%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20884-anti-cytochrome-c-antibody-7h82c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)IHC image of Cytochrome C staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13575, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
![Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20881-anti-cytochrome-c-antibody-7h82c12-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)Overlay histogram showing HepG2 cells stained with ab13575 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13575, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1]/mouse IgG2b [PLPV219] (ab91353/ab91366, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)](https://www.abcam.co.jp/ps/products/13/ab13575/Images/ab13575-20886-anti-cytochrome-c-antibody-7h82c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [7H8.2C12] (ab13575)Ab13575 staining human normal skin tissue. Staining is localised to mitochondria.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (175)
ab13575 は 175 報の論文で使用されています。
- Jia G et al. Neural stem cell-conditioned medium ameliorates Aß25-35-induced damage in SH-SY5Y cells by protecting mitochondrial function. Bosn J Basic Med Sci 21:179-186 (2021). PubMed: 32156251
- Jing L et al. Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells. BMC Complement Med Ther 21:18 (2021). PubMed: 33413359
- Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
- Liu L et al. The natural product lapiferin inhibits cell proliferation and promotes cell apoptosis in gingival squamous cell carcinoma via P21 regulation. Mol Med Rep 24:N/A (2021). PubMed: 33907850
- Devenport SN et al. Colorectal cancer cells utilize autophagy to maintain mitochondrial metabolism for cell proliferation under nutrient stress. JCI Insight 6:N/A (2021). PubMed: 34138755