Anti-Cytochrome C 抗体 [6H2.B4] (ab270249)

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Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (ab270249)
  • Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (ab270249)

Key features and details

  • Mouse monoclonal [6H2.B4] to Cytochrome C
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytochrome C antibody [6H2.B4]
    Cytochrome C 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [6H2.B4] to Cytochrome C

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IF
    Human
    See all applications and species data

  • 免疫原

    Full length protein within Rat Cytochrome C. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells. Flow cyt: HeLa cells.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IF
Human
All applications
Mouse
Rat
アプリケーション Abreviews 特記事項
IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
特記事項
IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.

ターゲット情報

  • 機能

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

  • 関連疾患

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

  • 配列類似性

    Belongs to the cytochrome c family.

  • 翻訳後修飾

    Binds 1 heme group per subunit.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

画像

  • Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (ab270249)
    Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (ab270249)

    Immunofluorescence analysis of HeLa cells labeling Cytochrome C (Green) using ab270249 at 2µg/ml, followed by goat anti-mouse IgG-CF488 (Green).

  • Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (ab270249)
    Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (ab270249)

    Flow cytometric analysis of PFA-fixed HeLa cells labeling cytochrome C using ab270249 at 2µg/106 cells followed by goat anti-mouse IgG-CF488 (Blue); goat anti-mouse IgG-CF488 is control (Red).

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab270249 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab270249 は論文での使用が確認できていません。

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