アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報
  1. Link

    cyp2c9-antibody-ab4236.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes
Share by email

Anti-CYP2C9 抗体 (ab4236)

  • Datasheet
Reviews (2)Q&A (5)References (6)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)

    Key features and details

    • Rabbit polyclonal to CYP2C9
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human
    • Isotype: IgG

    こちらの製品もご検討ください

    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    関連製品

    製品の概要

    • 製品名

      Anti-CYP2C9 antibody
      CYP2C9 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to CYP2C9
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Mouse, Human
    • 免疫原

      Recombinant full length protein corresponding to Human CYP2C9.

    • 特記事項


      The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

       This product was previously labelled as Cytochrome P450 2C9

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 8.00
      Constituent: 0.0536% PBS
    • Concentration information loading...
    • 精製度

      Ammonium Sulphate Precipitation
    • 一次抗体 備考

      The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cytochromes
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipases
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Metabolism
      • Types of disease
      • Cancer

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    アプリケーション

    Our Abpromise guarantee covers the use of ab4236 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
    • 配列類似性

      Belongs to the cytochrome P450 family.
    • 細胞内局在

      Endoplasmic reticulum membrane. Microsome membrane.
    • Target information above from: UniProt accession P11712 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1559 Human
      • Omim: 601130 Human
      • SwissProt: P11712 Human
      • Unigene: 282624 Human
      • 別名

        • (R)-limonene 6-monooxygenase antibody
        • (S)-limonene 6-monooxygenase antibody
        • (S)-limonene 7-monooxygenase antibody
        • CP2C9_HUMAN antibody
        • CPC9 antibody
        • CYP2C antibody
        • CYP2C10 antibody
        • CYP2C9 antibody
        • CYPIIC9 antibody
        • cytochrome P-450 S-mephenytoin 4-hydroxylase antibody
        • Cytochrome P-450MP antibody
        • Cytochrome P450 2C9 antibody
        • Cytochrome P450 MP-4 antibody
        • Cytochrome P450 MP-8 antibody
        • Cytochrome P450 PB-1 antibody
        • Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
        • Cytochrome p4502C9 antibody
        • flavoprotein-linked monooxygenase antibody
        • MGC149605 antibody
        • MGC88320 antibody
        • microsomal monooxygenase antibody
        • OTTHUMP00000020135 antibody
        • P450 MP antibody
        • P450 PB 1 antibody
        • P450 PB1 antibody
        • P450IIC9 antibody
        • P450MP antibody
        • S mephenytoin 4 hydroxylase antibody
        • S-mephenytoin 4-hydroxylase antibody
        • xenobiotic monooxygenase antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)
        IHC image of ab4236 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4236, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      プロトコール

      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
    • 参考文献 (6)

      ab4236 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab4236 は 6 報の論文で使用されています。

      • Sekretarska J  et al. Influence of Selected Carbon Nanostructures on the CYP2C9 Enzyme of the P450 Cytochrome. Materials (Basel) 12:N/A (2019). PubMed: 31835701
      • Guo R  et al. Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor. Stem Cell Reports 9:499-512 (2017). PubMed: 28757167
      • Cichocki JA  et al. Impact of Nonalcoholic Fatty Liver Disease on Toxicokinetics of Tetrachloroethylene in Mice. J Pharmacol Exp Ther 361:17-28 (2017). PubMed: 28148637
      • Skrypnyk N  et al. PPARa activation can help prevent and treat non-small cell lung cancer. Cancer Res 74:621-31 (2014). PubMed: 24302581
      • Irusta G  et al. Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum. Mol Hum Reprod 13:541-8 (2007). PubMed: 17567599
      • Vriens J  et al. Modulation of the Ca2 permeable cation channel TRPV4 by cytochrome P450 epoxygenases in vascular endothelium. Circ Res 97:908-15 (2005). PubMed: 16179585

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Question

      I am sending image of WB with the 2 antibodies I used:
      Anti-Cytochrome P450 4A11/22 antibody (ab110746)
      Anti-Cytochrome P450 2C9 antibody (ab4236)
      My WB protocol:
      Blocking - 5% milk/1 hour/ room temp
      Wash - t-tbs/5 min/ room temp
      Primary antibody - overnight/ 1:1000 in 5% milk/ 4C
      3x wash - t-tbs/5 min/ room temp
      Secondary antibody - 1 hour/ 1:10000 in 5% milk/ room temp
      3x wash - t-tbs/5 min/ room temp

      Read More

      Abcam community

      Verified customer

      Asked on Mar 06 2012

      Answer

      Thank you for your reply and for sending your data. I agree that the results you have obtained are difficult to interpret. For that reason, I would be happy to offer you free of charge replacements, or a credit or refund. Please let me know how you would like to proceed and I will be happy to assist you further.

      Read More

      Abcam Scientific Support

      Answered on Mar 06 2012

      Question

      Do you sell blocking peptides for ab4236 and ab110746?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 05 2012

      Answer

      Thank you for contacting us.
      Because ab4236 is a rabbit polyclonal antibody that was raised against a full length protein, you could use a full length recombinant Cytochrome P450 2C9 protein such as ab56998 for blocking. It is very difficult and expensive to achieve full blocking using a full length protein, however you may be able to observe a reduction in the specific signal by pre-incubation with this product. After checking with the lab, a blocking peptide for ab110746 is not currently available.
      I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

      Read More

      Abcam Scientific Support

      Answered on Mar 05 2012

      Question

      I would like to test ab4236 for use on rat samples.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: May 26th, 2012

      I am very pleased to hear you would like to accept our offer and test ab4236 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jan 26 2012

      Question

      To whom this may concern,   I would like to request for a Certificate of Compliance for each of the following antibodies.   Product No. ab4236 with Lot No. GR21040 Product No. ab22717 with Lot No. GR34397 Product No. ab62204 with Lot No. GR6576   Please send these certificates to this email address. Let me know if you have any questions or concerns regarding this email.  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 06 2011

      Answer

      Thank you for contacting Abcam. Please find attached the requested Certificate of Compliance

      Read More

      Abcam Scientific Support

      Answered on Dec 07 2011

      Western blot abreview for Anti-CYP2C9 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Sheep Tissue lysate - whole (Liver)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bis-Tris Gel)
      Loading amount
      80 µg
      Specification
      Liver
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Mr. Joseph Statwick

      Verified customer

      投稿 Mar 10 2011

      Western blot abreview for Anti-CYP2C9 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (liver)
      Gel Running Conditions
      Reduced Denaturing (4-20% gel)
      Loading amount
      30 µg
      Specification
      liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 13 2010

      Question

      What do you recommend as positive and negative controls for these antibodies?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 27 2006

      Answer

      Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 27 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      技術情報や製品情報をメールで受け取る 登録
      製品・研究分野別
      • 癌
      • 循環器
      • 細胞生物学
      • 発生
      • エピジェネティクス&核内情報
      • 免疫学
      • メタボリズム
      • 感染症
      • ニューロサイエンス
      • シグナル伝達
      • ステムセル
      製品・タイプ別
      • 一次抗体
      • 二次抗体
      • バイオケミカルズ
      • アイソタイプ・コントロール
      • マルチカラー・セレクター
      • キット
      • ローディング・コントロール
      • ライゼート
      • ペプチド
      • タンパク質
      • スライド
      • タグ抗体・細胞マーカー
      • その他試薬
      サービス&サポート
      • サポート
      • よくあるお問い合わせ
      • プロトコール&トラブルシュート
      • ご購入に関して
      • お問い合わせフォーム
      • トレーニング
      アブカムについて
      • 企業ウェブサイト
      • 投資家の皆様へ
      • 企業情報
      • 採用情報
      • アブカムについて
      イベント
      • 展示会
      • カンファレンス
      ブログ

      ブログを読む

      International websites
      • abcam.com
      • abcam.cn

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      © 1998-2021 Abcam plc. All rights reserved.