Anti-CYP27A1 抗体 [EPR7529] (ab126785)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7529] to CYP27A1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CYP27A1 antibody [EPR7529]
CYP27A1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7529] to CYP27A1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 6.00 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7529 -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/120.
|
|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF |
1/50 - 1/100.
|
特記事項 |
---|
Flow Cyt (Intra)
1/120. |
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
パスウェイ
Hormone biosynthesis; cholecalciferol biosynthesis. -
関連疾患
Cerebrotendinous xanthomatosis -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Entrez Gene: 301517 Rat
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- SwissProt: P17178 Rat
- Unigene: 516700 Human
see all -
別名
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
画像
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All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : Caco 2 lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
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Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab126785 は 31 報の論文で使用されています。
- Dong Z et al. Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced Steatosis. Cell Mol Gastroenterol Hepatol 13:1161-1179 (2022). PubMed: 34990887
- Wang T et al. Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35457188
- Qin W et al. Hepatic RACK1 deletion disturbs lipid and glucose homeostasis independently of insulin resistance. J Endocrinol 254:137-151 (2022). PubMed: 35608066
- Guo Y et al. Dihydroartemisinin promoted FXR expression independent of YAP1 in hepatocellular carcinoma. FASEB J 36:e22361 (2022). PubMed: 35616366
- Zhang Y et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. NPJ Sci Food 6:38 (2022). PubMed: 36030278