Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (5% gel)
Loading amount
5 µg
Treatment
Diluted in Laemmli buffer, boiled 90C for 7 minute
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 7.5% BSA, 1x TBS, 0.1% Tween-20 (TBST)
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: sc-2357: mouse anti-rabbit IgG-HRP (Santa Cruz Bio
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Loaded 5ug of mouse heart, red gastrocnemius, white adipose tissue, and liver (no CYFIP2 protein detected in these tissues)
Exposure
5 second(s)
Bands
Specific: 140 kDa Non-specific: N/A kDa
Positive control
Loaded 5ug, 10ug, 15ug, 20ug mouse brain
Additional data
Additional Notes
Polyvinylidene difluoride (PVDF) membrane
Lane Descriptions:
1: 5µg C57BL/6N mouse brain
2: 10µg C57BL/6N mouse brain
3: 15µg C57BL/6N mouse brain
4: 20µg C57BL/6N mouse brain
5: 5µg C57BL/6N mouse heart
6: 5µg C57BL/6N mouse red gastrocnemius
7: 5µg C57BL/6N mouse white adipose tissue
8: 5µg C57BL/6N mouse liver
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 05 2019