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遺伝子ノックアウト細胞株 検証済

Anti-Cyclophilin B 抗体 (ab16045)

  • Datasheet
  • SDS
Reviews (14)Q&A (11)References (90)

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Western blot - Anti-Cyclophilin B antibody (ab16045)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
  • Western blot - Anti-Cyclophilin B antibody (ab16045)
  • Western blot - Anti-Cyclophilin B antibody (ab16045)
  • Immunoprecipitation - Anti-Cyclophilin B antibody (ab16045)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
  • Western blot - Anti-Cyclophilin B antibody (ab16045)
  • Western blot - Anti-Cyclophilin B antibody (ab16045)

Key features and details

  • Rabbit polyclonal to Cyclophilin B
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Horse, Chicken, Dog, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cyclophilin B antibody
    Cyclophilin B 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Cyclophilin B
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details
  • 種交差性

    交差種: Mouse, Rat, Horse, Chicken, Dog, Human
    交差が予測される動物種: Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human Cyclophilin B aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16277, ab5016)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Immune Interventions
    • Transplantation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Isotype/Loading Controls
    • Loading Controls
    • Cyp-B

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Cyclophilin B protein (ab53870)
  • Recombinant Protein

    • Recombinant Cyclophilin B protein (ab53870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16045の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (7)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF (3)
Use a concentration of 1 µg/ml.
IP
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 関連疾患

    Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
  • 配列類似性

    Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • 細胞内局在

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P23284 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5479 Human
    • Entrez Gene: 19035 Mouse
    • Entrez Gene: 64367 Rat
    • Omim: 123841 Human
    • SwissProt: P24367 Chicken
    • SwissProt: P80311 Cow
    • SwissProt: P23284 Human
    • SwissProt: P24369 Mouse
    • SwissProt: P24368 Rat
    • Unigene: 434937 Human
    • Unigene: 335249 Mouse
    • Unigene: 1893 Rat
    see all
  • 別名

    • AA408962 antibody
    • AA553318 antibody
    • AI844835 antibody
    • Cphn 2 antibody
    • Cphn2 antibody
    • Cyclophilin B antibody
    • Cyclophilin like protein antibody
    • CyP 20b antibody
    • CYP S1 antibody
    • CYP-S1 antibody
    • CYPB antibody
    • EC 5.2.1.8 antibody
    • MGC14109 antibody
    • MGC2224 antibody
    • OI9 antibody
    • peptidyl prolyl cis trans isomerase B antibody
    • Peptidyl prolyl cis trans isomerase B precursor antibody
    • Peptidyl-prolyl cis-trans isomerase B antibody
    • peptidylprolyl isomerase B (cyclophilin B) antibody
    • Peptidylprolyl isomerase B antibody
    • PPIase antibody
    • PPIase B antibody
    • Ppib antibody
    • PPIB_HUMAN antibody
    • Rotamase antibody
    • Rotamase B antibody
    • S cyclophilin antibody
    • S-cyclophilin antibody
    • SCYLP antibody
    see all

画像

  • Western blot - Anti-Cyclophilin B antibody (ab16045)
    Western blot - Anti-Cyclophilin B antibody (ab16045)
    All lanes : Anti-Cyclophilin B antibody (ab16045) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PPIB knockout HAP1 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : U87-MG whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab16045 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab16045 was shown to specifically react with PPIB in wild-type HAP1 cells as signal was lost in PPIB knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. Ab16045 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)

    ab16045 stained HeLa cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16045 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-Cyclophilin B antibody (ab16045)
    Western blot - Anti-Cyclophilin B antibody (ab16045)
    All lanes : Anti-Cyclophilin B antibody (ab16045) at 1 µg/ml

    Lane 1 : Rat Liver
    Lane 2 : Mouse 3T3
    Lane 3 : Dog
    Lane 4 : Chicken

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Western blot - Anti-Cyclophilin B antibody (ab16045)
    Western blot - Anti-Cyclophilin B antibody (ab16045)
    All lanes : Anti-Cyclophilin B antibody (ab16045) at 1 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : HEK293 whole cell lysate
    Lane 6 : HeLa nuclear lysate with Human Cyclophilin B peptide (ab16277) at 1 µg/ml
    Lane 7 : HeLa whole cell lysate with Human Cyclophilin B peptide (ab16277) at 1 µg/ml
    Lane 8 : A431 whole cell lysate with Human Cyclophilin B peptide (ab16277) at 1 µg/ml
    Lane 9 : Jurkat whole cell lysate with Human Cyclophilin B peptide (ab16277) at 1 µg
    Lane 10 : HEK293 whole cell lysate with Human Cyclophilin B peptide (ab16277) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-Cyclophilin B antibody (ab16045)
    Immunoprecipitation - Anti-Cyclophilin B antibody (ab16045)
    Cyclophilin B was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cyclophilin B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16045.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 21kDa: Cyclophilin B.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)

    ICC/IF image of ab16045 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16045, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody (ab16045)This image is courtesy of an Abreview submitted by Dr Kirk McManus
    ab16045 (1/1000) staining Cyclophilin B in assynchronous HeLa cells (green). Cells were fixed with Paraformaldehyde and counter-stained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • Western blot - Anti-Cyclophilin B antibody (ab16045)
    Western blot - Anti-Cyclophilin B antibody (ab16045)Image courtesy of Dr M Schrader by Abreview.
    All lanes : Anti-Cyclophilin B antibody (ab16045) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from rat pancreatic AR42J cells, which were treated with 10nM dexamethasone for 48 hours.
    Lane 2 : Whole cell lysate for negative control, prepared from rat pancreatic AR42J cells (specific knock down of cyclophilin B/PpiB by siRNA), which were treated with 10nM dexamethasone for 48 hours.

    Secondary
    All lanes : Goat-anti-Rabbit HRP-conjugated polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Primary antibody incubated for 12 hours at 4°C.
    Blocking step performed using 5% milk, 1 hour at 20°C.

    See Abreview

  • Western blot - Anti-Cyclophilin B antibody (ab16045)
    Western blot - Anti-Cyclophilin B antibody (ab16045)
    Anti-Cyclophilin B antibody (ab16045) at 0.5 µg/ml + Recombinant Human Cyclophilin B protein (ab88801) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds

プロトコール

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (90)

ab16045 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16045 は 90 報の論文で使用されています。

  • Navarro-Guerrero E  et al. Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci Rep 11:4245 (2021). PubMed: 33608581
  • Lamarche É  et al. SMAD2 promotes myogenin expression and terminal myogenic differentiation. Development 148:N/A (2021). PubMed: 33462116
  • Melo HM  et al. Palmitate Is Increased in the Cerebrospinal Fluid of Humans with Obesity and Induces Memory Impairment in Mice via Pro-inflammatory TNF-a. Cell Rep 30:2180-2194.e8 (2020). PubMed: 32075735
  • Patel A  et al. Adefovir dipivoxil induces DNA replication stress and augments ATR inhibitor-related cytotoxicity. Int J Cancer 147:1474-1484 (2020). PubMed: 32159854
  • Johansson P  et al. A Patient-Derived Cell Atlas Informs Precision Targeting of Glioblastoma. Cell Rep 32:107897 (2020). PubMed: 32668248
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 25 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Cyclophilin B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton X-100 1%
Specification
Cerebellum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 03 2020

Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (M17 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
NTC and PPIB siRNA
Specification
M17 cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Nov 05 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Cyclophilin B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton x-100 1%
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 14 2020

Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 31 2020

Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hippocampal culture)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
15 µg
Specification
Hippocampal culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 27 2020

Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver extract)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Liver extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Stefano Benvegnu

Verified customer

投稿 Jan 20 2020

Western blot abreview for Anti-Cyclophilin B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Sheep Tissue lysate - whole (Placenta, caruncle)
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Loading amount
10 µg
Specification
Placenta, caruncle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

MISS. Barbara Makela

Verified customer

投稿 Oct 30 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclophilin B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (fibroblasts)
Specification
fibroblasts
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Dr. Shawna Pyott

Verified customer

投稿 Aug 24 2012

Question

human (HeLA), wrong bandsize: 40 kDa and cristal clear, no other band

Read More

Abcam community

Verified customer

Asked on Sep 19 2012

Answer

Vielen Dank für Ihre Anfrage.
Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und sollte morgen bei Ihnen ankommen.
Des Weiteren habe ich noch den Fragebogen angehängt, auf dass wir uns einen Überblick bezüglich des Protokolls machen können.
Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Abcam Scientific Support

Answered on Sep 19 2012

Question

will test ab16045 in horse

Read More

Abcam community

Verified customer

Asked on Aug 16 2012

Answer

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab16045 in horse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for horse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Abcam Scientific Support

Answered on Aug 16 2012

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