Anti-Cyclophilin B 抗体 (ab16045)

Vielen Dank für Ihre Anfrage.
Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und sollte morgen bei Ihnen ankommen.
Des Weiteren habe ich noch den Fragebogen angehängt, auf dass wir uns einen Überblick bezüglich des Protokolls machen können.
Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab16045 in horse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for horse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Thank you very much for your reply and for sending the images.

Please keep me updated about the results with the replacement vial, and let me know if there is anything else that we can do for you.

Have a nice day.

Thank you very much for your call today and for letting us know about the trouble with the new vial of this antibody.

As we discussed, I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow. Please keep me updated about the results with this new vial.

Also, we would be very appreciative if you could please send any images and a detailed protocol (including antibody dilution, blocking solution, etc) so that we can look into the problem with this recent vial.

I look forward to hearing from you. If you have any questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

Thank you for contacting us. I do hope the changes which we have talked about will help with your staining. As you have requested I have attached our ICC protocol which details alternative fixations to PFA. I have also created a 100% Abreview discount code for you. The code and additional information are below. I am very pleased to hear you would like to accept our offer and test ab16045 in Horse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Horse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting us.

I have tried to correlate the human housekeeping genes with chicken and then tried to find the whether these are expressed in Chicken heart. The following is the link to human housekeeping genes

http://www.cgen.com/supp_info/housekeeping_genes.html

Beta catenin is protein I have chose which is expressed in Chicken heart, evidence is in publicaiton Fig 5. http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0002857#pone-0002857-g004



The anti beta catenin antibodies are ab2365, ab23671,ab16051 and ab79089

Talin 1



Publication; http://www.biomedcentral.com/1471-2121/7/40

ab71333, ab95034

Aspartate aminotransferase



http://pubs.acs.org/doi/abs/10.1021/bi00841a018

ab59132, ab98228

Please note our recommendations are based on the data published in publications and Chicken tested antibodies. It is more likely that the antibodies we have might not have tested in chicken heart however as long as the protein is produced in heart the antibodies will be OK.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The two lysis buffer recipes in our western blotting guide, one for cytoskeletal protein lysis buffer and one for RIPA, are almost identical except for the addition of EDTA, EGTA, and glycerol in the cytoskeletal protein lysis buffer. I see no reason why the RIPA buffer would not recover as much protein as the cystoskeletal protein lysis buffer from chicken embryo heart.

Have you tried staining a blot of the heart homgenates prepared with the cystoskeletal protein lysis buffer? It is likely that this buffer is as effective as RIPA for recovering cyclophilin from your samples.

I do not think I can explain why the yield of total protein from RIPA is relatively low, if that is in fact the case. The other possibility I mentioned is the effect of the buffer reagents on the BCA assay, though as you have pointed out, they should all be within the acceptable limits of the assay. Are you confident the RIPA buffer was prepared correctly?

One way to check would be to prepare solutions of BSA in each buffer, of equal amounts by weight per volume, for example 1 mg/ml, and then compare the OD in the assay.

Thank you for contacting us.

Can you please tell me what your protein yield was with the cytoskeletal buffer? I am not sure why the RIPA yield would be much lower, unless your protein assay is incompatible with the RIPA buffer components. Can you please tell me what assay you use to determinethe protein concentration?