Application
ELISA
Sample
Rabbit Cell (rabbit liver cells)
Specification
rabbit liver cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
Type
Other
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP)
Additional data
Additional Notes
cyclophilin A (0.1 to 1 ug/mL PBS determined using protein assay kit ) or cell lysate (rabbit liver fresh cells were incubated with drugs for 24 hr, scraped, homogenized and protein content determined, dilution were made in PBS to have the same concentration than cyclophilin.) were coated for 3 hr at 37C
saturateion 2% BSA in PBS
anticyclophilin rabbit dilution 1/1000 in PBS 1% BSA
goat alkalline phosphatase 1/1000 in PBS 1% BSA
Washes: 3 times with PBS-Tween and 3 times with PBS (except before add of Sigma P7998 (alkaline phosphatase yellow liquid substrate system)
saturateion 2% BSA in PBS
anticyclophilin rabbit dilution 1/1000 in PBS 1% BSA
goat alkalline phosphatase 1/1000 in PBS 1% BSA
Washes: 3 times with PBS-Tween and 3 times with PBS (except before add of Sigma P7998 (alkaline phosphatase yellow liquid substrate system)
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投稿 Jul 28 2006