製品名Anti-Cyclophilin A antibody
Cyclophilin A 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Cyclophilin A
特異性Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
アプリケーション適用あり: ICC/IF, WB, ELISA, ICCmore details
種交差性交差種: Mouse, Rat, Rabbit, Hamster, Human
交差が予測される動物種: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab3563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
Used for 2 hrs on Rabbit liver cells.
|ICC||Use at an assay dependent concentration.|
機能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
配列類似性Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain.
- Information by UniProt
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : NIH-3T3
Lysates/proteins at 25 µg per lane.
Observed band size: 18 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Cowman S et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). Read more (PubMed: 30943920) »
- Chen J et al. Human Cytomegalovirus Encoded miR-US25-1-5p Attenuates CD147/EMMPRIN-Mediated Early Antiviral Response. Viruses 9:N/A (2017). Read more (PubMed: 29194430) »