Anti-Cyclophilin A 抗体 (ab3563)

Reviews (4)Q&A (7)References (10)
Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
  • Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
  • Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
  • Western blot - Anti-Cyclophilin A antibody (ab3563)
  • Western blot - Anti-Cyclophilin A antibody (ab3563)
  • Western blot - Anti-Cyclophilin A antibody (ab3563)
  • Western blot - Anti-Cyclophilin A antibody (ab3563)

Key features and details

  • Rabbit polyclonal to Cyclophilin A
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cyclophilin A antibody
    Cyclophilin A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cyclophilin A

  • 由来種

    Rabbit

  • 特異性

    Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey

  • 免疫原

    Recombinant full length protein corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.

  • ポジティブ･コントロール

    • WB: CyPA; A549, U-87 MG, Jurkat cells; NIH/3T3, HeLa, mouse lung, colon and stomach tissue; HepG2, K562. ICC/IF: A431, HeLa cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3563の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 1 µg/ml.
WB (2)
1/300.
特記事項
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/300.

ターゲット情報

  • 機能

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

  • 配列類似性

    Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
    Contains 1 PPIase cyclophilin-type domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cyclophilin A antibody
      • Cyclophilin antibody
      • CyclophilinA antibody
      • Cyclosporin A binding protein antibody
      • Cyclosporin A-binding protein antibody
      • CYPA antibody
      • CYPH antibody
      • Epididymis secretory sperm binding protein Li 69p antibody
      • HEL S 69p antibody
      • MGC117158 antibody
      • MGC12404 antibody
      • MGC23397 antibody
      • Peptidyl prolyl cis trans isomerase A antibody
      • Peptidyl-prolyl cis-trans isomerase A antibody
      • Peptidylprolyl isomerase A (cyclophilin A) antibody
      • Peptidylprolyl isomerase A antibody
      • PPIA antibody
      • PPIA protein antibody
      • PPIA_HUMAN antibody
      • PPIase A antibody
      • Rotamase A antibody
      • RotamaseA antibody
      • T cell cyclophilin antibody
      see all

    画像

    • Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
      Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)

      Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/250 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red Rhodamine Phalloidin, and the nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue). Negative control (e) is where no primary antibody was used.

    • Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
      Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)

      Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized HeLa cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).

    • Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
      Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)

      Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).

    • Western blot - Anti-Cyclophilin A antibody (ab3563)
      Western blot - Anti-Cyclophilin A antibody (ab3563)
      All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/100 dilution

      Lane 1 : NIH/3T3 whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : mouse lung tissue lysate
      Lane 4 : mouse colon tissue lysate
      Lane 5 : mouse stomach tissue lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 1/2500 dilution

      Developed using the ECL technique.
    • Western blot - Anti-Cyclophilin A antibody (ab3563)
      Western blot - Anti-Cyclophilin A antibody (ab3563)
      All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/3000 dilution

      Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
      Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, at 1/4000 dilution

      Developed using the ECL technique.
    • Western blot - Anti-Cyclophilin A antibody (ab3563)
      Western blot - Anti-Cyclophilin A antibody (ab3563)

      ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

    • Western blot - Anti-Cyclophilin A antibody (ab3563)
      Western blot - Anti-Cyclophilin A antibody (ab3563)
      All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : NIH-3T3

      Lysates/proteins at 25 µg per lane.

      Observed band size: 18 kDa why is the actual band size different from the predicted?

    参考文献 (10)

    ab3563 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3563 は 10 報の論文で使用されています。

    • Francis AC  et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11:3505 (2020). PubMed: 32665593
    • Ma-Lauer Y  et al. Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication. Antiviral Res 173:104620 (2020). PubMed: 31634494
    • Cowman S  et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). PubMed: 30943920
    • Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
    • Chen J  et al. Human Cytomegalovirus Encoded miR-US25-1-5p Attenuates CD147/EMMPRIN-Mediated Early Antiviral Response. Viruses 9:N/A (2017). PubMed: 29194430
    • Fan YN  et al. Mir-34a Mimics Are Potential Therapeutic Agents for p53-Mutated and Chemo-Resistant Brain Tumour Cells. PLoS One 9:e108514 (2014). WB . PubMed: 25250818
    • Lorenson MY  et al. A major prolactin-binding complex on human milk fat globule membranes contains cyclophilins A and B: the complex is not the prolactin receptor. Am J Physiol Endocrinol Metab 302:E585-94 (2012). WB ; Human . PubMed: 22205628
    • Waller H  et al. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 165:202-10 (2010). PubMed: 20132841
    • Kratochwill K  et al. Stress responses and conditioning effects in mesothelial cells exposed to peritoneal dialysis fluid. J Proteome Res 8:1731-47 (2009). PubMed: 19231869
    • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046

    レビューと Q&A

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    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-Cyclophilin A antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skin)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    skin
    Blocking step
    licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 23 2016

    Western blot abreview for Anti-Cyclophilin A antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MeT5a)
    Loading amount
    15 µg
    Specification
    MeT5a
    Gel Running Conditions
    Reduced Denaturing (10% Acrylamide, Laemmli buffer system)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 09 2008

    ELISA abreview for Anti-Cyclophilin A antibody

     Excellent
    Abreviews
    Application
    ELISA
    Sample
    Human Purified protein (competitive ELISA assay)
    Specification
    competitive ELISA assay
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
    Type
    Other
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 31 2006

    ELISA abreview for Anti-Cyclophilin A antibody

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Rabbit Cell (rabbit liver cells)
    Specification
    rabbit liver cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
    Type
    Other
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 28 2006

    Question

    Our lab is interested in purchasing ab3563. Can you provide us with the concentration of the antibody or the mass contained in one unit of this product?

    Read More
    Answer

    Thank you for your enquiry. This product ab3563 is supplied as 400 µl of total IgG in PBS containing 0.05% sodium azide. Unfortunately, the concentration of this product has not been determined at this time, as the antibody has only been protein (and not epitope affinity) purified. I am sorry I can not be of more assistance in this occasion but should you require any further help please do not hesitate to contact me.

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    Question

    What is the concentration of this product?

    Read More
    Answer

    Thank you for your enquiry. This antibody is listed on the data sheet as being an IgG fraction. Because this antibody is not affinity purified, the concentration is undetermined. I understand that you need the concentration for your assays, but unfortunately that information is unattainable. I apologize for any inconveniance this has caused you.

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    Question

    What is the concentration of ab3563?

    Read More
    Answer

    Thank you for your enquiry. Regarding polyclonal antibody to Cyclophilin A ab3563, our source has not measured the IgG concentration. The activity of some of the antibodies we sell is based on ELISA data. However,the concentration is expected to be between 1-2 mg/ml. I apologize for any inconvenience this may cause. Please let me know if I may provide further assistance.

    Read More

    Question

    Hi, do you reckon i could detect the CyPA in my cardia protein extracts to use it as a housekeeping? Thanks

    Read More
    Answer

    Thank you for your enquiry. Cyclophilin A is described as a housekeeoping gene in the literature. However, this cyclophilin A antibody does not detect endogenous levels of CyPA, so it would not be suitable for this purpose. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    I just purchased your product rabbit polyclonal to cyclophilin A(ab3563-400, \ Lot # 162382). Could you let me know the protein or IgG concentration of the antibody.

    Read More
    Answer

    I apologize, but it looks like the IgG concentration is unknown for ab3563 (Cyclophilin A). Occasionally, ELISA tests are used to determine activity instead of measuring the protein concentration. More than likely, the range falls between 0.5-2 mg/ml. Please contact us again if you have any additional questions.

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    Question

    BATCH NUMBER 75863 ORDER NUMBER 2005a285 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Human HeLa cell cell extract PRIMARY ANTIBODY ab3563 anti-Cyclophilin PAb 1:300 dilution for one hour, wash three times with TBST SECONDARY ANTIBODY anti-rabbit Ab-HRP/AP from Promega and Santa Cruz Biotech 1:10000 dilution for one hour, wash three times with TBST DETECTION METHOD Colormetric using Promega substrates either Western Blue or TMB stabilized depending on the secondary Ab used POSITIVE AND NEGATIVE CONTROLS USED None used ANTIBODY STORAGE CONDITIONS Stored at -20 d C in an enzyme cooler SAMPLE PREPARATION Heating sample in SDS buffer AMOUNT OF PROTEIN LOADED 1 x 10^5 cells per lane ELECTROPHORESIS/GEL CONDITIONS 4-20% SDS Page in Tris/Hepes/Glycine buffer TRANSFER AND BLOCKING CONDITIONS TBST with 0.5% dry milk at room temperature for 1 hr or 4 d C overnight HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No ADDITIONAL NOTES I used Ambion's anti-Cyclophlin A PAb in parallel with abcam's anti-CyPA and abcam's did not detect my target protein. I even applied [a competitor] anti-CyPA over a blotted abcam nitrocellulose membrane (when it could not detect it) and CyPA showed up after using Ambion's Ab.

    Read More
    Answer

    I'm sorry to hear you are experiencing problems with ab3563. I would like to recommend incubating the membrane in the antibody solution overnight at 4C. Please let me know if you still have problems following this recommendation and I will arrange a replacement to be sent to you.

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    1-10 of 11 Abreviews or Q&A

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