Anti-Cyclophilin A 抗体 (ab3563)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cyclophilin A antibody
Cyclophilin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclophilin A -
由来種
Rabbit -
特異性
Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
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免疫原
Recombinant full length protein corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.
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ポジティブ･コントロール
- WB: CyPA; A549, U-87 MG, Jurkat cells; NIH/3T3, HeLa, mouse lung, colon and stomach tissue; HepG2, K562. ICC/IF: A431, HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3563の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (2) |
1/300.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/300. |
ターゲット情報
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機能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
配列類似性
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
- Unigene: 356331 Human
- Unigene: 598115 Human
- Unigene: 5246 Mouse
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別名
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
画像
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Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/250 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red Rhodamine Phalloidin, and the nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue). Negative control (e) is where no primary antibody was used.
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Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized HeLa cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
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Immunocytochemistry - Anti-Cyclophilin A antibody (ab3563)Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
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Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/100 dilution
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : mouse lung tissue lysate
Lane 4 : mouse colon tissue lysate
Lane 5 : mouse stomach tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 1/2500 dilution
Developed using the ECL technique. -
Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, at 1/4000 dilution
Developed using the ECL technique. -
Western blot - Anti-Cyclophilin A antibody (ab3563)ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
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Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : NIH-3T3
Lysates/proteins at 25 µg per lane.
Observed band size: 18 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab3563 は 11 報の論文で使用されています。
- Storck SE et al. Brain endothelial LRP1 maintains blood-brain barrier integrity. Fluids Barriers CNS 18:27 (2021). PubMed: 34147102
- Francis AC et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11:3505 (2020). PubMed: 32665593
- Ma-Lauer Y et al. Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication. Antiviral Res 173:104620 (2020). PubMed: 31634494
- Cowman S et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). PubMed: 30943920
- Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
