Anti-Cyclophilin A 抗体 (ab3563)

Reviews (4)Q&A (7)References (9)
Western blot - Anti-Cyclophilin A antibody (ab3563)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
  • Western blot - Anti-Cyclophilin A antibody (ab3563)

Key features and details

  • Rabbit polyclonal to Cyclophilin A
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Hamster, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Cyclophilin A antibody
    Cyclophilin A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cyclophilin A

  • 由来種

    Rabbit

  • 特異性

    Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Hamster, Human
    交差が予測される動物種: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey

  • 免疫原

    Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300.

ターゲット情報

画像

  • Western blot - Anti-Cyclophilin A antibody (ab3563)
    Western blot - Anti-Cyclophilin A antibody (ab3563)

    ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
    ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Cyclophilin A antibody (ab3563)
    Western blot - Anti-Cyclophilin A antibody (ab3563)
    All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : NIH-3T3

    Lysates/proteins at 25 µg per lane.

    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

参考文献 (9)

ab3563 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3563 は 9 報の論文で使用されています。

  • Francis AC  et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11:3505 (2020). PubMed: 32665593
  • Cowman S  et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). PubMed: 30943920
  • Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
  • Chen J  et al. Human Cytomegalovirus Encoded miR-US25-1-5p Attenuates CD147/EMMPRIN-Mediated Early Antiviral Response. Viruses 9:N/A (2017). PubMed: 29194430
  • Fan YN  et al. Mir-34a Mimics Are Potential Therapeutic Agents for p53-Mutated and Chemo-Resistant Brain Tumour Cells. PLoS One 9:e108514 (2014). WB . PubMed: 25250818
  • Lorenson MY  et al. A major prolactin-binding complex on human milk fat globule membranes contains cyclophilins A and B: the complex is not the prolactin receptor. Am J Physiol Endocrinol Metab 302:E585-94 (2012). WB ; Human . PubMed: 22205628
  • Waller H  et al. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 165:202-10 (2010). PubMed: 20132841
  • Kratochwill K  et al. Stress responses and conditioning effects in mesothelial cells exposed to peritoneal dialysis fluid. J Proteome Res 8:1731-47 (2009). PubMed: 19231869
  • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046

レビューと Q&A

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1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Cyclophilin A antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
skin
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
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Abcam user community

Verified customer

投稿 Nov 23 2016

Western blot abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MeT5a)
Loading amount
15 µg
Specification
MeT5a
Gel Running Conditions
Reduced Denaturing (10% Acrylamide, Laemmli buffer system)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Sep 09 2008

Question

Our lab is interested in purchasing ab3563. Can you provide us with the concentration of the antibody or the mass contained in one unit of this product?

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Answer

Thank you for your enquiry. This product ab3563 is supplied as 400 µl of total IgG in PBS containing 0.05% sodium azide. Unfortunately, the concentration of this product has not been determined at this time, as the antibody has only been protein (and not epitope affinity) purified. I am sorry I can not be of more assistance in this occasion but should you require any further help please do not hesitate to contact me.

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Question

What is the concentration of this product?

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Answer

Thank you for your enquiry. This antibody is listed on the data sheet as being an IgG fraction. Because this antibody is not affinity purified, the concentration is undetermined. I understand that you need the concentration for your assays, but unfortunately that information is unattainable. I apologize for any inconveniance this has caused you.

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Question

What is the concentration of ab3563?

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Answer

Thank you for your enquiry. Regarding polyclonal antibody to Cyclophilin A ab3563, our source has not measured the IgG concentration. The activity of some of the antibodies we sell is based on ELISA data. However,the concentration is expected to be between 1-2 mg/ml. I apologize for any inconvenience this may cause. Please let me know if I may provide further assistance.

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ELISA abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
Application
ELISA
Sample
Human Purified protein (competitive ELISA assay)
Specification
competitive ELISA assay
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Type
Other
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Abcam user community

Verified customer

投稿 Jul 31 2006

ELISA abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Rabbit Cell (rabbit liver cells)
Specification
rabbit liver cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
Type
Other
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Abcam user community

Verified customer

投稿 Jul 28 2006

Question

Hi, do you reckon i could detect the CyPA in my cardia protein extracts to use it as a housekeeping? Thanks

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Answer

Thank you for your enquiry. Cyclophilin A is described as a housekeeoping gene in the literature. However, this cyclophilin A antibody does not detect endogenous levels of CyPA, so it would not be suitable for this purpose. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I just purchased your product rabbit polyclonal to cyclophilin A(ab3563-400, \ Lot # 162382). Could you let me know the protein or IgG concentration of the antibody.

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Answer

I apologize, but it looks like the IgG concentration is unknown for ab3563 (Cyclophilin A). Occasionally, ELISA tests are used to determine activity instead of measuring the protein concentration. More than likely, the range falls between 0.5-2 mg/ml. Please contact us again if you have any additional questions.

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Question

BATCH NUMBER 75863 ORDER NUMBER 2005a285 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Human HeLa cell cell extract PRIMARY ANTIBODY ab3563 anti-Cyclophilin PAb 1:300 dilution for one hour, wash three times with TBST SECONDARY ANTIBODY anti-rabbit Ab-HRP/AP from Promega and Santa Cruz Biotech 1:10000 dilution for one hour, wash three times with TBST DETECTION METHOD Colormetric using Promega substrates either Western Blue or TMB stabilized depending on the secondary Ab used POSITIVE AND NEGATIVE CONTROLS USED None used ANTIBODY STORAGE CONDITIONS Stored at -20 d C in an enzyme cooler SAMPLE PREPARATION Heating sample in SDS buffer AMOUNT OF PROTEIN LOADED 1 x 10^5 cells per lane ELECTROPHORESIS/GEL CONDITIONS 4-20% SDS Page in Tris/Hepes/Glycine buffer TRANSFER AND BLOCKING CONDITIONS TBST with 0.5% dry milk at room temperature for 1 hr or 4 d C overnight HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No ADDITIONAL NOTES I used Ambion's anti-Cyclophlin A PAb in parallel with abcam's anti-CyPA and abcam's did not detect my target protein. I even applied [a competitor] anti-CyPA over a blotted abcam nitrocellulose membrane (when it could not detect it) and CyPA showed up after using Ambion's Ab.

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Answer

I'm sorry to hear you are experiencing problems with ab3563. I would like to recommend incubating the membrane in the antibody solution overnight at 4C. Please let me know if you still have problems following this recommendation and I will arrange a replacement to be sent to you.

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1-10 of 11 Abreviews or Q&A

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