Anti-Cyclophilin 40 抗体 (ab3562)
Key features and details
- Rabbit polyclonal to Cyclophilin 40
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Non human primates
- Isotype: IgG
製品の概要
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製品名
Anti-Cyclophilin 40 antibody
Cyclophilin 40 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclophilin 40 -
由来種
Rabbit -
特異性
Detects cyclophilin 40 (CyP 40) from Human and Rat tissues and cells. This antibody does not cross-react with CyPA. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Chicken, Cow, Human, Non human primates -
免疫原
Synthetic peptide corresponding to Human Cyclophilin 40 aa 356-370.
Sequence:AQKDKEKAVYAKMFA
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Proprietary Purification -
一次抗体 備考
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | 1/1000. | |
IHC-P | Use at an assay dependent concentration. | |
ICC/IF | 1/200. |
ターゲット情報
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機能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 281420 Cow
- Entrez Gene: 5481 Human
- Entrez Gene: 67738 Mouse
- Entrez Gene: 361967 Rat
- Omim: 601753 Human
- SwissProt: P26882 Cow
- SwissProt: Q08752 Human
- SwissProt: Q9CR16 Mouse
see all -
別名
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
画像
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ab3562 at a dilution of 1/1000 staining Cyp 40 in Rat spleen lysate by Western blot.
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Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/Immunofluorescence analysis of A431 cells labeling Cyclophilin 40 (green) with ab3562 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at aab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Cyclophilin D (ab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
プロトコール
データシートおよび資料
参考文献 (6)
ab3562 は 6 報の論文で使用されています。
- Yim KH et al. Gambogic acid identifies an isoform-specific druggable pocket in the middle domain of Hsp90ß. Proc Natl Acad Sci U S A 113:E4801-9 (2016). PubMed: 27466407
- Zhu G et al. Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment. Oncol Lett 12:3478-3484 (2016). WB ; Human . PubMed: 27900024
- Xu XD et al. The study of energy metabolism in bladder cancer cells in co-culture conditions using a microfluidic chip. Int J Clin Exp Med 8:12327-36 (2015). PubMed: 26550142
- Allan AM et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . PubMed: 24755652
- Han J et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct hax-1 cleavage. J Biol Chem 285:22461-72 (2010). WB ; Human . PubMed: 20388708
- Trauernicht AM et al. Modulation of estrogen receptor alpha protein level and survival function by DBC-1. Mol Endocrinol 21:1526-36 (2007). PubMed: 17473282