Anti-Cyclin T1 抗体 (ab2098)

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Key features and details

  • Rabbit polyclonal to Cyclin T1
  • Suitable for: WB, IP, ELISA, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Cyclin T1 antibody
    Cyclin T1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cyclin T1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ELISA, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Fusion protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
ELISA 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.

ターゲット情報

  • 機能

    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

  • 組織特異性

    Ubiquitously expressed.

  • 配列類似性

    Belongs to the cyclin family. Cyclin C subfamily.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)

    ab2098 diluted 1:500 to detect Cyclin T1 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
    ab2098 (2µg/ml) staining Cyclin T1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Western blot - Anti-Cyclin T1 antibody (ab2098)
    Western blot - Anti-Cyclin T1 antibody (ab2098)
    Anti-Cyclin T1 antibody (ab2098) at 1/10000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 48 kDa, 78 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes


    The 90 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Cyclin T1.

参考文献

ab2098 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2098 は 12 報の論文で使用されています。

  • Bidaux G  et al. FRET Image Correlation Spectroscopy Reveals RNAPII-Independent P-TEFb Recruitment on Chromatin. Biophys J 114:522-533 (2018). PubMed: 29414698
  • Sakurai N  et al. BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene. Sci Rep 7:11962 (2017). PubMed: 28931940
  • Lefèvre L  et al. Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of ß-catenin in adrenocortical carcinoma. Oncogenesis 4:e161 (2015). ICC/IF . PubMed: 26214578
  • Muniz L  et al. RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP. Nucleic Acids Res 41:4686-98 (2013). Human . PubMed: 23471002
  • Luo Z  et al. The super elongation complex family of RNA polymerase II elongation factors: gene target specificity and transcriptional output. Mol Cell Biol 32:2608-17 (2012). PubMed: 22547686
View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Question

BATCH NUMBER 115294 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM more bands, unspecific SAMPLE Cell extract PRIMARY ANTIBODY Abcam, ab2098,Rabbit polyclonal 1:1000, 1h RT, wash 5 times a 5 min with Western wash DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED no, but other batches from you always worked fine ANTIBODY STORAGE CONDITIONS aliquoted a 20ul in -20 SAMPLE PREPARATION Ripa Buffer, Protese Inhibotors AMOUNT OF PROTEIN LOADED 20-50ug ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 10% TRANSFER AND BLOCKING CONDITIONS 1 hour blocking in 5% BSA in Western Wash Buffer SECONDARY ANTIBODY Jackson Immuno Research :Goat anti rabbit IgG-HRP 1:10.000, 1 hr, RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 20 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? nothing altered, since we got fine results with other batch (we are out of this Antidody)

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been experiencing definitely appear to be batch related. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

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Question

Customer's enquiry: Is the immunogen sequence available?

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Answer

Sorry, the sequence is not available.

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Question

Is 85 mg/ml correct?

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Answer

The protein concentration is 85 mg/ml.

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Question

I am interested in using either cyclin T1-ab2098 or cdk9-ab6544 in a chromatin immunoprecipitation assay using human B cells to identify binding by P-TEFb (PITALRE) in vivo. Have either antibody been used in this assay and which would you recommend trying of the two? Many thanks Dr. Julian knight

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Answer

Neither of these antibodies has yet been tested for ChIP, although they work very well in standard IP. We are unable to suggest which of the two may be better. However, we would be very interested to hear the results and will reward you with an amazon.com gift certificate ($30) for you data. Good luck with your experiments,

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