アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報
  1. Link

    cyclin-d1-antibody-sp4-ab16663.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cyclins Cyclin D Family
Share by email
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Cyclin D1 抗体 [SP4] (ab16663)

  • Datasheet
  • SDS
Reviews (18)Q&A (5)References (176)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
  • Anti-Cyclin D1 antibody [SP4] (ab16663)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP4] to Cyclin D1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

ノックアウト
Product image
Human CCND1 (Cyclin D1) knockout HeLa cell line (ab255348)
ノックアウト
Product image
Human CCND1 (Cyclin D1) knockout HeLa cell line (ab261760)
一次抗体
Product image
Anti-Cdk4 antibody [EPR2513Y] (ab68266)

関連製品

製品の概要

  • 製品名

    Anti-Cyclin D1 antibody [SP4]
    Cyclin D1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [SP4] to Cyclin D1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • エピトープ

    C-terminus
  • ポジティブ・コントロール

    • WB: MCF7, Hap1, A431 and HeLa, Nuero-2a, NIH/3T3, C6 cell lysates. IHC (FFPE): Human normal tonsil; breast carcinoma; mantle cell lymphoma; rat esophagus. ICC/IF: MCF7 cells, C6, Neuro-2a and HAP1 cells (HAP1-CCND1 knockout cells used as negative cell line). Flow Cyt: MCF7, NIH/3T3 and C6 cells.
  • 特記事項

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product was switched from a hybridoma to recombinant production method on 22nd January 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    SP4
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin D Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin D Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin D family

関連製品

  • Alternative Versions

    • Anti-Cyclin D1 antibody [SP4] - BSA and Azide free (ab239794)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CCND1 (Cyclin D1) knockout HeLa cell line (ab255348)
    • Human CCND1 (Cyclin D1) knockout HeLa cell line (ab261760)
  • KO cell lysates

    • Human CCND1 (Cyclin D1) knockout HeLa cell lysate (ab256864)
    • Human CCND1 (Cyclin D1) knockout HeLa cell lysate (ab263808)
  • Recombinant Protein

    • Recombinant Human Cyclin D1 protein (ab85247)
  • Related Products

    • Recombinant Human Cyclin D1 protein (ab85247)

アプリケーション

Our Abpromise guarantee covers the use of ab16663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/250.
WB 1/25 - 1/200. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.

Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Primary Antibody Incubation: Incubate for 30 minutes at room temperature.

Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.

Flow Cyt 1/30.

ターゲット情報

  • 機能

    Essential for the control of the cell cycle at the G1/S (start) transition.
  • 関連疾患

    Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
    Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
    Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
  • 配列類似性

    Belongs to the cyclin family. Cyclin D subfamily.
  • 翻訳後修飾

    Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
    Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P24385 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 595 Human
    • Entrez Gene: 12443 Mouse
    • Entrez Gene: 58919 Rat
    • Omim: 168461 Human
    • SwissProt: P24385 Human
    • SwissProt: P25322 Mouse
    • SwissProt: P39948 Rat
    • Unigene: 523852 Human
    • Unigene: 667996 Human
    • Unigene: 273049 Mouse
    • Unigene: 22279 Rat
    see all
  • 別名

    • AI327039 antibody
    • B cell CLL/lymphoma 1 antibody
    • B cell leukemia 1 antibody
    • B cell lymphoma 1 protein antibody
    • B-cell lymphoma 1 protein antibody
    • BCL 1 antibody
    • BCL-1 antibody
    • BCL-1 oncogene antibody
    • BCL1 antibody
    • BCL1 oncogene antibody
    • ccnd1 antibody
    • CCND1/FSTL3 fusion gene antibody
    • CCND1/FSTL3 fusion gene, included antibody
    • CCND1/IGHG1 fusion gene antibody
    • CCND1/IGHG1 fusion gene, included antibody
    • CCND1/IGLC1 fusion gene antibody
    • CCND1/IGLC1 fusion gene, included antibody
    • CCND1/PTH fusion gene antibody
    • CCND1/PTH fusion gene, included antibody
    • CCND1_HUMAN antibody
    • cD1 antibody
    • Cyl 1 antibody
    • D11S287E antibody
    • G1/S specific cyclin D1 antibody
    • G1/S-specific cyclin-D1 antibody
    • Parathyroid adenomatosis 1 antibody
    • PRAD1 antibody
    • PRAD1 oncogene antibody
    • U21B31 antibody
    see all

画像

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    All lanes : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CCND1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab16663 observed at 36 kDa. Red - Anti-Vinculin antibody [VIN-54] observed at 124 kDa.

     ab16663 was shown to react with CCND1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255348 (knockout cell lysate ab263808) was used. Wild-type HeLa and CCND1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab16663 and Anti-Vinculin antibody [VIN-54] overnight at 4°C at a 1 in 200 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)

    IHC image of ab16663 staining Cyclin D1 in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab16663, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was generated from the hybridoma version.

     

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution + Wild-type HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab16663 observed at 36 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

     ab16663 was shown to react with Cyclin D1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261760 (knockout cell lysate ab256864) was used. Wild-type HeLa and CCND1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab16663 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 200 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling Cyclin D1 with purified ab16663 at 1/50 (5.42µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Flow cytometry analysis of MCF-7 (human breast carcinoma) labeling Cyclin D1 with purified ab16663 at 1/30 dilution (9.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

    This image was generated from the hybridoma version.

     

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    All lanes : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
    Lane 3 : CCND1 KO HAP1 cell lysate
    Lane 4 : HAP1 (Human chronic myelogenous leukemia near-haploid cell line) cell lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
    Lane 7 : C6 (Rat glial tumor glial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 2 seconds
  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    All lanes : Anti-Cyclin D1 antibody [SP4] (ab16663)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CCND1 (Cyclin D1) knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 33 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab16663 observed at 34 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab16663 was shown to specifically recognize CCND1 (Cyclin D1) in wild-type HAP1 cells as signal was lost at the expected MW in CCND1 (Cyclin D1) knockout cells. Wild-type and CCND1 (Cyclin D1) knockout samples were subjected to SDS-PAGE. Ab16663 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/200 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    This image was generated from the hybridoma version.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)

    IHC image of ab16663 staining Cyclin D1 in rat esophagus formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16663, 1:100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Cyclin D1µ with purified ab16663 at 1/50 (5.42µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)

    ab16663 staining Cyclin D1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab16663 at a working dilution of 1/250 and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-rabbit AlexaFluor® 488 (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Flow cytometry analysis of C6 (rat glioma) labeling Cyclin D1 with purified ab16663 at 1/30 dilution (9.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -  Unlabelled cells (blue).

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Flow Cytometry - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling Cyclin D1 with purified ab16663 at 1/30 dilution (9.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] (ab16663)

    ab16663 staining Cyclin D1 in wild-type HAP1 cells (top panel) and CCND1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab16663 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was generated from the hybridoma version.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)Image from McIver SC et al., PLoS One. 2012;7(4):e35553. Epub 2012 Apr 20. Fig 7.; doi:10.1371/journal.pone.0035553; April 20, 2012, PLoS ONE 7(4): e35553.

    Immunohistochemical analysis of mouse testis tissue, staining Cyclin D1 with ab16663.

    Antigen retrieval was performed via Tris-EDTA buffer. Sections were blocked with 3% BSA and incubated with primary antibody (1/50) overnight at 4°C. An AlexaFluor®594-conjugated secondary antibody was used to detect staining.

    This image was generated from the hybridoma version.

     

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Anti-Cyclin D1 antibody [SP4] (ab137875) at 1/5000 dilution + MCF-7 cell lysate

    Predicted band size: 33 kDa



    This image was generated from the hybridoma version.

     

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
    Lane 1 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution
    Lane 2 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/400 dilution

    All lanes : Whole cell lysate prepared from T24 bladder cancer cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG conjugated to HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 10 minutes


    Gel run under denaturing conditions 4-12% gradient.

    This image was generated from the hybridoma version.

     

    See Abreview

  • Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Western blot - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/25 dilution + MCF7 cell lysate

    Predicted band size: 33 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    This image was generated from the hybridoma version.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder

    ab16663 staining Cyclin D1 in Human urinary tract tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    This image was generated from the hybridoma version.

     

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [SP4] (ab16663)

    Human mantle cell lymphoma stained with ab16663.

    This image was generated from the hybridoma version.

     

  • Anti-Cyclin D1 antibody [SP4] (ab16663)
    Anti-Cyclin D1 antibody [SP4] (ab16663)

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (176)

    ab16663 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16663 は 176 報の論文で使用されています。

    • Qin Y  et al. Sonic hedgehog signaling pathway in Myelodysplastic Syndrome: Abnormal activation and jervine intervention. Gene 754:144881 (2020). PubMed: 32526259
    • Li C  et al. Circ_0008035 contributes to cell proliferation and inhibits apoptosis and ferroptosis in gastric cancer via miR-599/EIF4A1 axis. Cancer Cell Int 20:84 (2020). PubMed: 32190008
    • Cai P  et al. MicroRNA-107 may regulate lung cancer cell proliferation and apoptosis by targeting TP53 regulated inhibitor of apoptosis 1. Oncol Lett 19:1958-1966 (2020). PubMed: 32194690
    • Rong Z  et al. Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression. J Exp Clin Cancer Res 39:61 (2020). PubMed: 32272940
    • Tarang S  et al. Spatiotemporally controlled overexpression of cyclin D1 triggers generation of supernumerary cells in the postnatal mouse inner ear. Hear Res 390:107951 (2020). PubMed: 32244147
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 23 Abreviews or Q&A

    Western blot abreview for Anti-Cyclin D1 antibody [SP4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW480 cells)
    Loading amount
    25 µg
    Specification
    SW480 cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 24 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [SP4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (organ cultured corneal epithelium)
    Specification
    organ cultured corneal epithelium
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 1% Triton
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Read More

    Dr. Zhiguo He

    Verified customer

    投稿 Nov 06 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin D1 antibody [SP4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (esophagus)
    Specification
    esophagus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
    Permeabilization
    No
    Blocking step
    DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
    Read More

    Dr. J Chai

    Verified customer

    投稿 Apr 16 2012

    Western blot abreview for Anti-Cyclin D1 antibody [SP4]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    40 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More

    Dr. Robert Kupp

    Verified customer

    投稿 Sep 17 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [SP4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RPE1-hTERT cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    RPE1-hTERT cells
    Blocking step
    10% FBS, 1% BSA, 0.1% Triton X-100, 0.01% NaN3 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin D1 antibody [SP4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Urogenital Sinus (Fetal Prostate))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
    Permeabilization
    No
    Specification
    Urogenital Sinus (Fetal Prostate)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 02 2018

    Western blot abreview for Anti-Cyclin D1 antibody [SP4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Epithelial OVCAR cell line)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    100 µg
    Treatment
    Perifosine 0/12/24h
    Specification
    Epithelial OVCAR cell line
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 21 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin D1 antibody [SP4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat Placenta)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Rat Placenta
    Blocking step
    Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 26 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin D1 antibody [SP4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citrate buffer
    Permeabilization
    No
    Specification
    Colon adenocarcinoma
    Blocking step
    (agent) for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 08 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [SP4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (KMS12, U266, RPMI-8266, Daudi)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    KMS12, U266, RPMI-8266, Daudi
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Ms. Natalie Erdmann

    Verified customer

    投稿 Sep 10 2015

    1-10 of 23 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.