Anti-Cyclin D1 抗体 (ab226977)
Key features and details
- Rabbit polyclonal to Cyclin D1
- Suitable for: WB, IP, ICC/IF, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cyclin D1 antibody
Cyclin D1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin D1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, Sandwich ELISAmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat, Cow, Dog, Rhesus monkey -
免疫原
Recombinant full length protein corresponding to Human Cyclin D1.
Database link: P24385 -
ポジティブ・コントロール
- WB: HeLa, MCF7 and MDA-MB-231 whole cell extracts. IP: MCF7 whole cell extract. ICC/IF: SK-N-SH cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab226977の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 34 kDa.
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IP |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/1000.
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Sandwich ELISA |
1/1000 - 1/10000.
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特記事項 |
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WB
1/500 - 1/10000. Predicted molecular weight: 34 kDa. |
IP
1/100 - 1/500. |
ICC/IF
1/100 - 1/1000. |
Sandwich ELISA
1/1000 - 1/10000. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G1/S (start) transition. -
関連疾患
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
配列類似性
Belongs to the cyclin family. Cyclin D subfamily. -
翻訳後修飾
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 524530 Cow
- Entrez Gene: 449028 Dog
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Entrez Gene: 58919 Rat
- Entrez Gene: 574320 Rhesus monkey
- Omim: 168461 Human
- SwissProt: Q2KI22 Cow
see all -
別名
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
画像
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All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Cyclin D1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 34 kDa12% SDS-PAGE gel.
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All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 34 kDa12% SDS-PAGE
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4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling Cyclin D1 (green) using ab226977 at 1/500 dilution in ICC/IF.
Counterstain: Cytoskeleton marker beta Tubulin 3/ Tuj1 antibody.
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Cyclin D1 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226977. Western blot was performed from the immunoprecipitate using ab226977. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab226977 IP in MCF7 whole cell extract.
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Sandwich ELISA of recombinant full-length Cyclin D1 protein using ab226977 at 5μg/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (106)
ab226977 は 106 報の論文で使用されています。
- Wang S et al. Knockdown of circ_0004585 enhances the chemosensitivity of colorectal cancer cells to 5-fluorouracil via the miR-874-3p/CCND1 axis. Histol Histopathol 38:99-112 (2023). PubMed: 35900059
- Chen J et al. S-3'-hydroxy-7', 2', 4'-trimethoxyisoxane, a novel ferroptosis inducer, promotes NSCLC cell death through inhibiting Nrf2/HO-1 signaling pathway. Front Pharmacol 13:973611 (2022). PubMed: 36105203
- Han Z et al. DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway. Front Oncol 12:1036401 (2022). PubMed: 36408174
- Pan Q et al. CircSAMD11 facilitates progression of cervical cancer via regulating miR-503/SOX4 axis through Wnt/β-catenin pathway. Clin Exp Pharmacol Physiol 49:175-187 (2022). PubMed: 34546569
- Xu X et al. Proteomics analysis reveals the correlation of programmed ROS-autophagy loop and dysregulated G1/S checkpoint with imatinib resistance in chronic myeloid leukemia cells. Proteomics 22:e2100094 (2022). PubMed: 34564948