Anti-Cyclin B2/CCNB2 抗体 [X29.2] (ab18250)
Key features and details
- Mouse monoclonal [X29.2] to Cyclin B2/CCNB2
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Cyclin B2/CCNB2 antibody [X29.2]
Cyclin B2/CCNB2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [X29.2] to Cyclin B2/CCNB2 -
由来種
Mouse -
特異性
Reacts with most cyclin Bs. -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Xenopus laevis Cyclin B2/CCNB2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
X29.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Accumulates steadily during G2 and is abruptly destroyed at mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 9133 Human
- Omim: 602755 Human
- SwissProt: O95067 Human
- Unigene: 194698 Human
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別名
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
see all
画像
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IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab18250 は 18 報の論文で使用されています。
- Zhou Z et al. lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis. Aging (Albany NY) 13:9838-9858 (2021). PubMed: 33744866
- Benot-Dominguez R et al. Olive leaf extract impairs mitochondria by pro-oxidant activity in MDA-MB-231 and OVCAR-3 cancer cells. Biomed Pharmacother 134:111139 (2021). PubMed: 33360155
- Huang S et al. Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma. Aging (Albany NY) 12:3025-3041 (2020). PubMed: 32045367
- Wang X et al. Effect of miR-205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism. Oncol Lett 19:2568-2574 (2020). PubMed: 32194761
- Lin J et al. Exportin-T promotes tumor proliferation and invasion in hepatocellular carcinoma. Mol Carcinog 58:293-304 (2019). PubMed: 30334580