Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品名Anti-Cyclin B1 (phospho S126) antibody
Cyclin B1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Cyclin B1 (phospho S126)
特異性Minimal reactivity is expected with the non-phosphorylated form of the protein.
アプリケーション適用あり: WBmore details
交差が予測される動物種: Mouse, Rat, Hamster, Dog, Chimpanzee
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
精製度Immunogen affinity purified
一次抗体 備考This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Our Abpromise guarantee covers the use of ab3488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
機能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
配列類似性Belongs to the cyclin family. Cyclin AB subfamily.
発生段階Accumulates steadily during G2 and is abruptly destroyed at mitosis.
翻訳後修飾Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
ab3488 は 1 報の論文で使用されています。
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297