Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Treatment
double thymidine synchronization
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 58 kDa
Additional data
Additional Notes
lane 1: 0 hr after released from thymidine block
lane 2-9: 8-15 hrs after released from thymidine block
Signal is strong and also correlates with cell cycle progression, peaks in mitosis. Very good anti-cyclinB1 antibody.
lane 2-9: 8-15 hrs after released from thymidine block
Signal is strong and also correlates with cell cycle progression, peaks in mitosis. Very good anti-cyclinB1 antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 14 2009