Anti-Cyclin A2 抗体 [E67.1] (ab39)
製品の概要
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製品名
Anti-Cyclin A2 antibody [E67.1]
Cyclin A2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [E67.1] to Cyclin A2 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, ICC, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Cow, Human, African green monkey -
免疫原
Full length protein (Cow).
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エピトープ
The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1 -
特記事項
Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E67.1 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab39 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. PubMed: 18077453 | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. | |
ICC | Use at an assay dependent concentration. | |
IP | Use a concentration of 2.5 µg/ml. | |
IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
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参照データベース
- Entrez Gene: 281667 Cow
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Omim: 123835 Human
- SwissProt: P30274 Cow
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
see all -
別名
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
画像
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Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E67.1] (ab39)Image courtesy of an anonymous Abreview.ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Sahara N et al. Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain. J Alzheimers Dis 33:249-63 (2013). Read more (PubMed: 22941973) »
- Gao Z & Godbout R Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal 23:555-65 (2011). WB . Read more (PubMed: 21111810) »