Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
200 µg
Specification
293T
Immuno-precipitation step
Protein G
Other product details
Concentration
4 µg/mg lysate
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA Buffer
Additional data
Additional Notes
This antibody can immunoprecipitate Cyclin A from both Human (293T) and Rat (PC12) cell extract. The species assigned as Cyclin A runs at ~55 kDa.
Lane order:
1-3 = 293T: 1, Cyclin A IP 2, Con IP 3, Input 10%
4-6 = PC12: 4, Cyclin A IP 5, Con IP 3, Input 10%
Lane order:
1-3 = 293T: 1, Cyclin A IP 2, Con IP 3, Input 10%
4-6 = PC12: 4, Cyclin A IP 5, Con IP 3, Input 10%
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投稿 Nov 13 2007