Key features and details
- Mouse monoclonal [E23.1] to Cyclin A2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品名Anti-Cyclin A2 antibody [E23.1]
Cyclin A2 一次抗体 製品一覧
製品の詳細Mouse monoclonal [E23.1] to Cyclin A2
アプリケーション適用あり: IHC-P, WBmore details
種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow
Fusion protein corresponding to Cow Cyclin A2.
- In Western Blot, ab38 gavre a positive signal in the following whole cell lysates: HEK293; U20S; PC12; NIH3T3. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab38 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).|
機能Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
配列類似性Belongs to the cyclin family. Cyclin AB subfamily.
発生段階Accumulates steadily during G2 and is abruptly destroyed at mitosis.
細胞内局在Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
IHC image of Cyclin A staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/ml
Lane 1 : U2OS (Hu) whole cell lysate
Lane 2 : 293T (Hu) whole cell lysate
Lane 3 : Cos-7 (AGMk) whole cell lysate
Lane 4 : CHO (Hm) whole cell lysate
Lane 5 : PC12 (Rat) whole cell lysate
Lane 6 : 3T3 (Ms) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated goat anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.
Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.
ab38 は 46 報の論文で使用されています。
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- Tang W et al. MicroRNA-29b-3p inhibits cell proliferation and angiogenesis by targeting VEGFA and PDGFB in retinal microvascular endothelial cells. Mol Vis 26:64-75 (2020). PubMed: 32165827
- Zhang L et al. Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways. Oncol Rep 42:1843-1855 (2019). PubMed: 31432177
- Moran-Salvador E et al. Fibrogenic Activity of MECP2 Is Regulated by Phosphorylation in Hepatic Stellate Cells. Gastroenterology 157:1398-1412.e9 (2019). PubMed: 31352003
- Fragkos M et al. Dicer prevents genome instability in response to replication stress. Oncotarget 10:4407-4423 (2019). PubMed: 31320994