Anti-Cyclin A1 抗体 (ab53699)
Key features and details
- Rabbit polyclonal to Cyclin A1
- Suitable for: ICC/IF, ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cyclin A1 antibody
Cyclin A1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin A1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, ELISA, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. -
組織特異性
Very high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8900 Human
- Entrez Gene: 12427 Mouse
- Omim: 604036 Human
- SwissProt: P78396 Human
- SwissProt: Q61456 Mouse
- Unigene: 417050 Human
- Unigene: 4815 Mouse
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別名
- CCN A1 antibody
- CCNA 1 antibody
- Ccna1 antibody
see all
画像
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All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution
Lane 1 : SKOV3 cell extract
Lane 2 : SKOV3 cell extract with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (34)
ab53699 は 34 報の論文で使用されています。
- Kim W et al. Suppression of transient receptor potential melastatin 7 regulates pluripotency, proliferation, and differentiation of mouse embryonic stem cells via mechanistic target of rapamycin-extracellular signal-regulated kinase activation. J Cell Biochem 123:547-567 (2022). PubMed: 34958137
- Zhang R et al. The lncRNA DANCR promotes development of atherosclerosis by regulating the miR-214-5p/COX20 signaling pathway. Cell Mol Biol Lett 27:15 (2022). PubMed: 35177003
- Zhuang J et al. Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner. BMC Cardiovasc Disord 22:69 (2022). PubMed: 35196979
- Ko J et al. Protective Effect of GIP against Monosodium Glutamate-Induced Ferroptosis in Mouse Hippocampal HT-22 Cells through the MAPK Signaling Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35204073
- Deng Y et al. RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1. Biol Proced Online 24:4 (2022). PubMed: 35321657