Western blot abreview for Anti-CXCR4 antibody [UMB2]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney cells (clear cell renal carcinoma))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
Kidney cells (clear cell renal carcinoma)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBS Tween (0.1%)
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Dako Anti-Rabbit Secondary
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Clear cell renal carcinoma parental cells (786-O) not expressing CXCR4
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Positive control
Clear cell renal carcinoma metastatic clones (M1A) expressing CXCR4

Additional data

Additional Notes
Do not boil protein samples prior to loading in the gel. Super signal ECL Femto Maximum Sensitivity substrate was required
PubMed ID
29875134: View

Paulo Rodrigues

Verified customer

投稿 Aug 10 2018

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