Immunohistochemistry (Frozen sections) abreview for Anti-CXCR4 antibody [UMB2]

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Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.2% triton TX-100
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBS
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: donkey anti-rabbit Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568

Additional data

Additional Notes
ABTRIAL 2% PFA/PBS by perfusion 30 min and then fixed the heart en bloc for 2 hrs in 2% PFA. Embedded in OCT. Co-stain with DAPI

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投稿 Feb 24 2016

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