Western blot abreview for Anti-CXCR4 antibody [UMB2]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (BEL-7404)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Treatment
80µM Doxorubicin for 24hrs
Specification
BEL-7404
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: cell signaling technology
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 39 kDa

Dr. guoqing zhu

Verified customer

投稿 Jan 20 2016

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