Western blot abreview for Anti-CXCR4 antibody [UMB2]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10% Invitrogen gel)
Loading amount
40 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA/PBST
Dilution
1/250

Secondary antibody

Dilution
1/5000

Detection

Detection method
Odyssey IR
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 41 kDa
Positive control
Lysed WT C57BL/6 mouse heart samples

Additional data

Additional Notes
Images show an alpha-tubulin loading control (green). I have grey scale of just the CXCR4 bands or red only channel if you want it.

Mr. Dan Bromage

Verified customer

投稿 Dec 30 2015

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