Immunocytochemistry/ Immunofluorescence abreview for Anti-CXCR4 antibody [UMB2]

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (1BR3.G fibroblast)
Permeabilization
Yes - Triton 1%
Specification
1BR3.G fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: rt°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: 2% BSA / PBS
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Anti Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
CXCR4 staining is located in nuclei and cytoplasm. We do not believe it as protein is a trans-membrane receptor ... Doesn't give better signal even more concentrated. Also tried with methanol with identical negative results... Nuclear staining with DAPI in blue. CXCR4 in green channel.
Abcam response
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投稿 Jul 22 2015

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