Western blot abreview for Anti-CXCR4 antibody [UMB2]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (HEK239)
Gel Running Conditions
Reduced Denaturing
Loading amount
100000 cells
Treatment
transfected with cxcr4 (mouse) plasmid or transfected with the empty expression vector
Specification
HEK239
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/50000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 42, 47 kDa

Additional data

Additional Notes
(1) HEK293 cells transfected with a cxcr4 (mouse) expression vector (2) HEK293 cells transfected with an empty expression vector Comments: The lysates were not boiled before loading. A nitrocellulose membrane was used. Two bands were observed, which is probably due to posttranslational modifications. The negative control shows the amount of endogenous CXCR4 in HEK293 cells.

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投稿 Nov 19 2013

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