(1) HEK293 cells transfected with a cxcr4 (mouse) expression vector
(2) HEK293 cells transfected with an empty expression vector
Comments: The lysates were not boiled before loading. A nitrocellulose membrane was used. Two bands were observed, which is probably due to posttranslational modifications. The negative control shows the amount of endogenous CXCR4 in HEK293 cells.