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Our Abpromise guarantee covers the use of ab1670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18369102
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ Immunofluorescence analysis of mouse bronchoalveolar lavage cells labeling CXCR4 with ab1670 at 1/200 dilution. Cells were fixed in formaldehyde and permeabilized with 1% triton X100 for 5 minutes. A polyclonal rabbit anti-goat Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fetal brain tissue labeling CXCR4 with ab1670 at 4 µg/ml. Tissue was fixed in formaldehyde; 1%BSA was used to block tissue for 30 minutes at 25°C. Heat mediated antigen retrieval was performed using a citrate buffer. A polyclonal rabbbit anti-goat IgG Biotin conjugated secondary antibody was used at 1/500 dilution. A good staining of neural cells was observed.
Flow Cytometry analysis of cells from mouse bone marrow (C57b6) labeling CXCR4 with ab1670 at 1/50 dilution. A polyclonal dobkey anti-goat DyLight® 649 secondary antibody was used at 1/1000 dilution. Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis. Cell population was gated on live cells (DAPI-), blood cells were excluded.
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