Anti-CXCR3 抗体 (ab71864)
Key features and details
- Rabbit polyclonal to CXCR3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CXCR3 antibody
CXCR3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCR3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.
-
ポジティブ・コントロール
- This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: HeK293 cell line
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71864の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).
|
|
ICC/IF |
Use a concentration of 1 µg/ml.
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11. -
組織特異性
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 2833 Human
- Entrez Gene: 12766 Mouse
- Entrez Gene: 84475 Rat
- Omim: 300574 Human
- SwissProt: P49682 Human
- SwissProt: O88410 Mouse
- SwissProt: Q9JII9 Rat
- Unigene: 198252 Human
see all -
別名
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD182 antibody
see all
画像
-
ICC/IF image of ab71864 stained HEK293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesOur observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab71864 は 3 報の論文で使用されています。
- SRI 2022: Scientific Abstracts. Reprod Sci 29:1-335 (2022). PubMed: 35181845
- Guan C et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). PubMed: 29456788
- O'Beirne SL et al. CXCL9 Regulates TGF-ß1-Induced Epithelial to Mesenchymal Transition in Human Alveolar Epithelial Cells. J Immunol 195:2788-96 (2015). PubMed: 26268659