Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4450(2)] to CXCL5 - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
製品名Anti-CXCL5 antibody [EPR4450(2)] - BSA and Azide free
CXCL5 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4450(2)] to CXCL5 - BSA and Azide free
アプリケーション適用あり: ICC/IF, IP, WBmore details
適用なし: Flow Cyt or IHC-P
Synthetic peptide within Human CXCL5 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P42830
ab248173 is the carrier-free version of ab126763 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248173 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 9.50 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa).|
機能Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
翻訳後修飾N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
All lanes : Anti-CXCL5 antibody [EPR4450(2)] (ab126763) at 1/1000 dilution
Lane 1 : A549 cell lysate, treated with TNF alpha and TPA at 10 µg
Lane 2 : A549 cell lysate, untreated at 10 µg
Lane 3 : Human CXCL5 recombinant protein at 0.001 µg
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa
This data was developed using ab126763, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.