Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human pancreas and colon)
Total protein in input
5000 µg
Specification
human pancreas and colon
Immuno-precipitation step
Protein G
Other product details
Dilution
1/2500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Western Blot antibody
None used
Additional data
PubMed ID
21248225: View
Additional Notes
Detection was performed through an Selected Reaction Monitoring approach using an Agilent 6460 QqQ Mass Spectrometer. Detailed IP conditions and a similar detection method was described in paper "Mutant proteins as cancer-specific biomarkers" (www.pnas.org/content/108/6/2444.short).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Qing Wang
Verified customer
投稿 Aug 02 2011