Anti-CXCL2 抗体 [EPR23331-159] (ab275879)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23331-159] to CXCL2
- Suitable for: IP, WB
- Reacts with: Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-CXCL2 antibody [EPR23331-159]
CXCL2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23331-159] to CXCL2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Rat lung organ (treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant ;Recombinant Rat CXCL2 protein. IP: Rat lung (organ treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23331-159 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab275879の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 11 kDa).
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特記事項 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 114105 Rat
- SwissProt: P30348 Rat
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別名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
画像
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All lanes : Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution
Lane 1 : Untreated rat lung organ culture, supernatant
Lane 2 : Rat lung organ (treated with 10/ml Concanavalin A for 3 days) culture, supernatant
Lysates/proteins at 10 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
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CXCL2 was immunoprecipitated from 0.35 mg Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant with ab275879 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275879 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant 10 μl
Lane 2: ab275879 IP in Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275879 in rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
This blot was developed using a higher sensitivity ECL substrate.
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Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution + Recombinant Rat CXCL2 protein, 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab275879 は論文での使用が確認できていません。