Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
製品の状態Lyophilized:Reconstitute with 200µl of sterile water.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab9841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
Use 100 µl/well antibody solution. Detects of 0.2 - 0.4 ng/well of recombinant human CXCL2.
機能Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
翻訳後修飾The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
- Information by UniProt
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
To detect human CXCL2 by Western Blot analysis ab9841 can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human CXCL2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant CXCL2 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant CXCL2 protein.
To detect human CXCL2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9841 is required. This antigen affinity purified antibody, in conjunction with anti-CXCL2 (Biotin) (ab245843) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CXCL2 .