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CX3CR1 peptide (ab8125)

Reviews (1)Q&A (1)References (1)
Western blot - CX3CR1 peptide (ab8125)

    Key features and details

    • Suitable for: Blocking, WB

    製品の詳細

    特性

    Our Abpromise guarantee covers the use of ab8125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Blocking

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituent: 0.1% BSA

    関連情報

    • 別名

      • Beta chemokine receptor-like 1
      • C X3 C CKR 1
      • C-X3-C CKR-1
      • CCRL1
      • Chemokine C X3 C motif receptor 1
      • CMK BRL 1
      • CMK-BRL-1
      • CMK-BRL1
      • CMKBLR1
      • CMKDR1
      • CX3C chemokine receptor 1
      • CX3C CKR1
      • CX3C1_HUMAN
      • CX3CR1
      • Fractalkine receptor
      • G-protein coupled receptor 13
      • GPR13
      • GPRV28
      • V28
      see all

    • 機能

      Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

    • 組織特異性

      Expressed in lymphoid and neural tissues.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    画像

    • Western blot - CX3CR1 peptide (ab8125)
      Western blot - CX3CR1 peptide (ab8125)
      All lanes : CX3CR1 antibody

      Lane 1 : Human spleen lysate
      Lane 2 : Human spleen lysate with CX3CR1 peptide (ab8125)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab8125 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8125 は 1 報の論文で使用されています。

    • Shen F  et al. Novel Small-Molecule CX3CR1 Antagonist Impairs Metastatic Seeding and Colonization of Breast Cancer Cells. Mol Cancer Res 14:518-27 (2016). PubMed: 27001765

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Other (Immunohistochemistry peptide block) Abreview for CX3CR1 peptide (Human)

     Excellent
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: Used as a control for blocking the activity of anti-CX3CR1 (ab8021) in immunohistochemistry frozen sections. Peptide was incubated with an equal volume of antibody at 37*C for 30 mins before applying to sections diluted 1:400 in PBS.
    Sample: Mouse Tissue sections

    Primary antibody (in addition to 'CX3CR1 peptide (Human)')
    Primary antibody: Abcam primary antibody: Anti-CX3CR1 antibody (ab8021)
    Dilution: 1/400

    Secondary antibody
    Name: Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) *2 mg/
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Alexa Fluor® 568
    Dilution: 1/800

    Abcam user community

    Verified customer

    投稿 Nov 30 2010

    Question

    Dear technical team, Our customer purchased ab8021 and stained xxxxx and observed with confocal. She got good results but her professor doesn't believe this data. There's no reference CX3CR1 detected in this tissue untilnow by his knowledge. So, the customer would like to askhow she canprove this data is true. I hope you can help our customer. Best regards,

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    Answer

    Thank you for contacting us. I am happy to hear that your customer has exciting results. I suggest to include additional control to verify these results: The blocking peptide can be used to show that the antibody binds specifically. If the antibody is incubated with the blocking peptide before incubating the samples, the specific staining will not appear (compare to slide without blocking peptide). ab8125 (CX3CR1 peptide) Click here (or use the following: https://www.abcam.com/CX3CR1-peptide-ab8125.html). I can also recommend to run positive and negative controls along side the actual sample. If still in doubt, I suggest to use an additional antibody against CX3CR1 that was raised against a different immunogen. If both show the same staining your customer has a strong case to believe their results. Please find all our CX3CR1 antibodies by following this link: https://www.abcam.com/index.html?pageconfig=searchresults I hope this information is helpful for your customer. Please do not hesitate to contact me again with any further questions in this matter.

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