CX3CR1 peptide (ab8125)

Reviews (1)Q&A (1)

製品の詳細

特性

Our Abpromise guarantee covers the use of ab8125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA

関連情報

  • 別名

    • Beta chemokine receptor-like 1
    • C X3 C CKR 1
    • C-X3-C CKR-1
    • CCRL1
    • Chemokine C X3 C motif receptor 1
    • CMK BRL 1
    • CMK-BRL-1
    • CMK-BRL1
    • CMKBLR1
    • CMKDR1
    • CX3C chemokine receptor 1
    • CX3C CKR1
    • CX3C1_HUMAN
    • CX3CR1
    • Fractalkine receptor
    • G-protein coupled receptor 13
    • GPR13
    • GPRV28
    • V28
    see all

  • 機能

    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

  • 組織特異性

    Expressed in lymphoid and neural tissues.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

画像

  • Western blot - CX3CR1 peptide (ab8125)
    Western blot - CX3CR1 peptide (ab8125)
    All lanes : CX3CR1 antibody

    Lane 1 : Human spleen lysate
    Lane 2 : Human spleen lysate with CX3CR1 peptide (ab8125)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献

ab8125 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8125 は論文での使用が確認できていません。

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Dear technical team, Our customer purchased ab8021 and stained xxxxx and observed with confocal. She got good results but her professor doesn't believe this data. There's no reference CX3CR1 detected in this tissue untilnow by his knowledge. So, the customer would like to askhow she canprove this data is true. I hope you can help our customer. Best regards,

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Answer

Thank you for contacting us. I am happy to hear that your customer has exciting results. I suggest to include additional control to verify these results: The blocking peptide can be used to show that the antibody binds specifically. If the antibody is incubated with the blocking peptide before incubating the samples, the specific staining will not appear (compare to slide without blocking peptide). ab8125 (CX3CR1 peptide) Click here (or use the following: https://www.abcam.com/index.html?datasheet=8125). I can also recommend to run positive and negative controls along side the actual sample. If still in doubt, I suggest to use an additional antibody against CX3CR1 that was raised against a different immunogen. If both show the same staining your customer has a strong case to believe their results. Please find all our CX3CR1 antibodies by following this link: https://www.abcam.com/index.html?pageconfig=searchresults I hope this information is helpful for your customer. Please do not hesitate to contact me again with any further questions in this matter.

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Other (Immunohistochemistry peptide block) Abreview for CX3CR1 peptide (Human)

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Abreviews
abreview image
Application
Other

Review text: Used as a control for blocking the activity of anti-CX3CR1 (ab8021) in immunohistochemistry frozen sections. Peptide was incubated with an equal volume of antibody at 37*C for 30 mins before applying to sections diluted 1:400 in PBS.
Sample: Mouse Tissue sections

Primary antibody (in addition to 'CX3CR1 peptide (Human)')
Primary antibody: Abcam primary antibody: Anti-CX3CR1 antibody (ab8021)
Dilution: 1/400

Secondary antibody
Name: Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) *2 mg/
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution: 1/800

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投稿 Nov 30 2010

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