Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CX3CR1 antibody

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Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat spinal cord with lesion)
Specification
Rat spinal cord with lesion
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 5% BSA and 0.1% Triton-X 100
Dilution
1/200

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
Rat L5 spinal cord was lesioned on one side. The CX3CR1-labeled microglia was significantly induced compared to the un-lesion control side.

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投稿 Jan 03 2015

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