製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CX3CR1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 2-21 of Human CX3CR1 (Peptide available as ab8125.)

  • ポジティブ・コントロール
    • Human spleen tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • 組織特異性
    Expressed in lymphoid and neural tissues.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

画像

  • Mouse heart tissue stained for CX3CR1 using ab8021 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-CX3CR1 antibody (ab8021)

    Lane 1 : Human spleen lysate with absence of blocking peptide.
    Lane 2 : Human spleen lysate with presence of blocking peptide.

    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml.
  • Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.

  • Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml. 

    Green: Isotype control

    Red: CX3CR1 antibody (ab8021) 

  • Anti-CX3CR1 antibody (ab8021) at 1 µg/ml + Recombinant Human CX3CR1 protein (ab112276) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 minutes

参考文献

This product has been referenced in:
  • Wang J  et al. Ischemia-induced Neuronal Cell Death Is Mediated by Chemokine Receptor CX3CR1. Sci Rep 8:556 (2018). Read more (PubMed: 29323156) »
  • Balzano T  et al. The Cerebellum of Patients with Steatohepatitis Shows Lymphocyte Infiltration, Microglial Activation and Loss of Purkinje and Granular Neurons. Sci Rep 8:3004 (2018). Read more (PubMed: 29445232) »

See all 34 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Frozen Heart Sections (10um thick sections))
Permeabilization
Yes - 0.3% Trition for 30mins at RT
Specification
Frozen Heart Sections (10um thick sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 May 17 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat spinal cord with lesion)
Specification
Rat spinal cord with lesion
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
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投稿 Jan 03 2015

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (human microglia cell induced from monocyte)
Specification
human microglia cell induced from monocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
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投稿 Jan 03 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification
Rat spinal cord with lesion on different days
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 May 28 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Mouse Cell (BM)
Specification
BM
Gating Strategy
monocyte
Permeabilization
No
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投稿 Apr 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP-1 cells)
Specification
THP-1 cells
Fixative
Acetone
Permeabilization
No
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投稿 Jul 27 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cardiac tissue)
Permeabilization
No
Specification
Cardiac tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Acetone
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投稿 Nov 25 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin wound)
Specification
Skin wound
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
None
Username

Mr. Josh Meisner

Verified customer

投稿 Aug 19 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7 Macrophage Cell Line)
Permeabilization
Yes - PBS
Specification
RAW 264.7 Macrophage Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Sep 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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