製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to CX3CR1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human CX3CR1 aa 2-21 (N terminal).
    Database link: P49238
    (Peptide available as ab8125)

  • ポジティブ・コントロール

    • Western Blot: Human spleen tissue lysate, A431, A549, Caco-2, Daudi, HeLa, THP-1 cell lysates. ICC/IF: Human heart cells. IHC-P: Mouse, rat and human heart tissue. Flow cytometry: THP-1 cells. IF: Mouse and rat heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 0.1 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • 組織特異性

    Expressed in lymphoid and neural tissues.
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在

    Cell membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

画像

  • All lanes :

    Lane 1 : A431 cell lysates
    Lane 2 : A549 cell lysates
    Lane 3 : Caco-2 cell lysates
    Lane 4 : Daudi cell lysates
    Lane 5 : HeLa cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Developed using the ECL technique.


    10% gel.

    Running conditions: 130v for 2 hours.

    Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).

    Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.

    Primary antibody incubation: Room temperature for 1 hour.

    Secondary antibody incubation: Room temperature for 1 hour. 

    Washing conditions: 15 mL TSBT, 3 x 10 minutes. 

    Exposure: ECL solution

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling CX3CR1 with ab8021 at 2 µg/mL.

  • Immunofluorescence of CX3CR1 in mouse heart tissue with ab8021 at 20 ug/ml. Green: ab8021 Blue: DAPI staining

  • All lanes :

    All lanes : THP-1 cell line

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Developed using the ECL technique.


    10% gel.

    Running conditions: 130v for 2 hours.

    Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).

    Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.

    Primary antibody incubation: Room temperature for 1 hour.

    Secondary antibody incubation: Room temperature for 1 hour. 

    Washing conditions: 15 mL TSBT, 3 x 10 minutes. 

    Exposure: ECL solution

  • All lanes : Anti-CX3CR1 antibody (ab8021) at 1 µg/ml

    Lane 1 : Human spleen lysate with absence of blocking peptide.
    Lane 2 : Human spleen lysate with presence of blocking peptide.

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Mouse heart tissue stained for CX3CR1 using ab8021 at 2 µg/ml in immunohistochemical analysis.

  • Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml. 

    Green: Isotype control

    Red: CX3CR1 antibody (ab8021) 

  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml.
  • Immunofluorescence analysis of rat heart tissue labeling CX3CR1 with ab8021 at 2 µg/mL. DAPI stain in blue. 

  • Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.

  • Anti-CX3CR1 antibody (ab8021) at 1 µg/ml + Recombinant Human CX3CR1 protein (ab112276) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 minutes

参考文献

This product has been referenced in:

  • Tao T  et al. Modulating cAMP responsive element binding protein 1 attenuates functional and behavioural deficits in rat model of neuropathic pain. Eur Rev Med Pharmacol Sci 23:2602-2611 (2019). Read more (PubMed: 30964189) »
  • Wang J  et al. Ischemia-induced Neuronal Cell Death Is Mediated by Chemokine Receptor CX3CR1. Sci Rep 8:556 (2018). Read more (PubMed: 29323156) »
See all 40 Publications for this product

レビューと Q&A

1-10 of 19 Abreviews or Q&A

Question
Answer

Thank you for contacting us. The alignment of the ab8021 immunogen sequence with the protein at the following link showed 90% identity, so we expect the antibody will react with your samples.

http://www.ncbi.nlm.nih.gov/protein/355559765?report=genbank&log$=protalign&blast_rank=20&RID=RTCPUT7M01N

The code we discussed will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rhesus macaque and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 May 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Kidney
Blocking step
Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Mr. Joshua Dodge

Verified customer

投稿 Jan 22 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Frozen Heart Sections (10um thick sections))
Permeabilization
Yes - 0.3% Trition for 30mins at RT
Specification
Frozen Heart Sections (10um thick sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 May 17 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat spinal cord with lesion)
Specification
Rat spinal cord with lesion
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 03 2015

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (human microglia cell induced from monocyte)
Specification
human microglia cell induced from monocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 03 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification
Rat spinal cord with lesion on different days
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Jan 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

投稿 May 28 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Mouse Cell (BM)
Specification
BM
Gating Strategy
monocyte
Permeabilization
No

Abcam user community

Verified customer

投稿 Apr 25 2014

Question
Answer

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

This antibody was tested for flow cytometry in an outside laboratory, and all of the protocol details are not available. It was diluted 1:1000 for FC using CX3CR1-transfected cells, so the antibody may need to be used at a higher concentrated for non-transfected cells. We would recommend our standard blocking agent, 10% FCS, though other sera should be fine as well (except for rabbit serum, as this will results in high background from the anti-rabbit secondary antibody).

I am sorry that I don't have more information, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

Read More

1-10 of 19 Abreviews or Q&A

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