Anti-CX3CR1 抗体 (ab7201)

Submit a review Q&A (2)References (7)
Western blot - Anti-CX3CR1 antibody (ab7201)

    Key features and details

    • Rabbit polyclonal to CX3CR1
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CX3CR1 antibody
      CX3CR1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to CX3CR1

    • 由来種

      Rabbit

    • 特異性

      Recombinant and natural human and rat CX3CR1.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat, Human

    • 免疫原

      Recombinant amino terminal fragment (Human).

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7201 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/1000 - 1/2000.

    ターゲット情報

    • 機能

      Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

    • 組織特異性

      Expressed in lymphoid and neural tissues.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Beta chemokine receptor-like 1 antibody
        • C X3 C CKR 1 antibody
        • C-X3-C CKR-1 antibody
        • CCRL1 antibody
        • Chemokine C X3 C motif receptor 1 antibody
        • CMK BRL 1 antibody
        • CMK-BRL-1 antibody
        • CMK-BRL1 antibody
        • CMKBLR1 antibody
        • CMKDR1 antibody
        • CX3C chemokine receptor 1 antibody
        • CX3C CKR1 antibody
        • CX3C1_HUMAN antibody
        • CX3CR1 antibody
        • Fractalkine receptor antibody
        • G-protein coupled receptor 13 antibody
        • GPR13 antibody
        • GPRV28 antibody
        • V28 antibody
        see all

      画像

      • Western blot - Anti-CX3CR1 antibody (ab7201)
        Western blot - Anti-CX3CR1 antibody (ab7201)
        Western blot detection of CX3CR1 in the Brain by rabbit polyclonal to human CX3CR1 (V28). Western blot detection of CX3CR1 in the Brain by rabbit polyclonal to human CX3CR1 (V28).

      参考文献 (7)

      ab7201 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab7201 は 7 報の論文で使用されています。

      • Song CH  et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011). PubMed: 21813601
      • Jamieson WL  et al. CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism. Cancer Res 68:1715-22 (2008). Human . PubMed: 18339851
      • Yang XP  et al. Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway. Circ Res 101:1001-8 (2007). PubMed: 17885215
      • Zhang T  et al. Preferential involvement of CX chemokine receptor 4 and CX chemokine ligand 12 in T-cell migration toward melanoma cells. Cancer Biol Ther 5:1304-12 (2006). PubMed: 16929176
      • Hannan NJ  et al. The chemokines, CX3CL1, CCL14, and CCL4, promote human trophoblast migration at the feto-maternal interface. Biol Reprod 74:896-904 (2006). IHC-P ; Human . PubMed: 16452465
      • Zhang T  et al. CXC chemokine ligand 12 (stromal cell-derived factor 1 alpha) and CXCR4-dependent migration of CTLs toward melanoma cells in organotypic culture. J Immunol 174:5856-63 (2005). PubMed: 15843590
      • Hannan NJ  et al. Coexpression of fractalkine and its receptor in normal human endometrium and in endometrium from users of progestin-only contraception supports a role for fractalkine in leukocyte recruitment and endometrial remodeling. J Clin Endocrinol Metab 89:6119-29 (2004). PubMed: 15579768

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      1-2 of 2 Abreviews or Q&A

      Question

      Hello I want to purchase a WB Ab for human CX3CR1 to verify that my siRNA works. Which of the 4 products you have is best to order for this application (ab8021, ab7200, ab7201, ab51668)? Thank you

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      Answer

      Thank you for your enquiry and your interest in our products. All of these 4 antibodies (ab8021, ab7200, ab7201, ab51668) recognize CX3CR1 and are suitable for Western blot application. However, ab7200 has been tested and characterized for detecting rat proteinonly - and not human. The immunogen used to raise this antibody was a recombinant and natural rat CX3CR1 and we do not have any data yet if ab7200 would recognize the human form. It seems that ab8021 is the most popular amongst these antibodies and there are several Abreviews and specific publications listed on the product datasheet. You may wish to take a careful look at it before making the final decision: https://www.abcam.com/cx3cr1-antibody-ab8021.html As you may be aware, RT-PCR data should be treated carefully since, there is no clear correlation between mRNA and protein levels and sometimes it is not easy to predict the protein expression levels from quantitative mRNA data. There are several publications available which delineate the technical boundaries of the approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient. For this reason, I would suggest running a good positive control along with the samples on the same gel; for this purpose tissue lysate from human spleen can be applied. I hope this helps and if I can assist further, please do not hesitate to contact me.

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      Question

      I am looking to buy an antibody against mouse CX3CR1 to be used for IHC-Fr. Please can you advise whether either ab7201 has recently been tested on mouse or whether ab8020 has been used successfully for IHC-Fr.

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      Answer

      Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab7201. Also, ab8020 has not yet been tested for application in IHC-frozen sections (the antibody has only so far been tested in Western blotting). Should you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

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