Anti-Cullin 4A/CUL-4A 抗体 [EPR3198] - BSA and Azide free (ab247585)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free
Cullin 4A/CUL-4A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen used for this product shares 93% homology with Cul4B. Cross-reactivity with Cul4B has been observed using overexpression vectors but not on endogenous samples.
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アプリケーション
適用あり: IHC-P, IP, WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, A375, 293T, HEK-293 transfected with human full length Cul4A and Cul4B expression vectors whole cell lysates. IHC-P: Human breast carcinoma, Hepatocellular carcinoma, Colonic adenocarcinoma, Ovarian carcinoma tissue. IP: HeLa.
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特記事項
ab247585 is the carrier-free version of ab92554.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3198 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa. |
ターゲット情報
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機能
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the cullin family. -
翻訳後修飾
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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参照データベース
- Entrez Gene: 8451 Human
- Omim: 603137 Human
- SwissProt: Q13619 Human
- Unigene: 339735 Human
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別名
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
see all
画像
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All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/10000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) transfected with blank vectors whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4B expression vectors whole cell lysate
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4A expression vectors whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 104,88 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Ab92554 recognizes human Cul4A and Cul4B overexpression vectors.
ab180602 was used as a loading control.
This data was developed using ab92554, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/20000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDaThis data was developed using ab92554, the same antibody clone in a different buffer formulation.
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This data was developed using ab92554, the same antibody clone in a different buffer formulation.
Cullin 4A/CUL-4A was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab92554 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab92554 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab92554 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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This data was developed using ab92554, the same antibody clone in a different buffer formulation.ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247585 は論文での使用が確認できていません。