Anti-CTNNBL1 抗体 [EP2669Y] (ab76243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2669Y] to CTNNBL1
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CTNNBL1 antibody [EP2669Y]
CTNNBL1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2669Y] to CTNNBL1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa cell lysate, MCF7 cell lysate IHC-P: Human breast tissue ICC/IF: HeLa cells Flow Cyt: HeLa cells
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特記事項
This product has switched from a hybridoma to recombinant production method on 18th September 2023
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2669Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76243の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/20.
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WB |
1/5000. Predicted molecular weight: 65 kDa.
For the unpurified version use at 1/2000 to 1/5000 dilution |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For the unpurified version use at 1/100 to 1/250 dilution |
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ICC/IF |
1/50.
|
特記事項 |
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Flow Cyt
1/20. |
WB
1/5000. Predicted molecular weight: 65 kDa. For the unpurified version use at 1/2000 to 1/5000 dilution |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For the unpurified version use at 1/100 to 1/250 dilution |
ICC/IF
1/50. |
ターゲット情報
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機能
Induces apoptosis in CHO cells. -
組織特異性
Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56259 Human
- Omim: 611537 Human
- SwissProt: Q8WYA6 Human
- Unigene: 472667 Human
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別名
- 5730471K09Rik antibody
- Beta catenin like 1 antibody
- Beta catenin like protein 1 antibody
see all
画像
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Flow cytometry analysis showing positive staining in HeLa cells using ab76243 at 1:20 dilution, 5 µg/mL.
Cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab76243 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.
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HeLa cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab76243 at 1:50 was incubated overnight at 4° C, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab76243 overnight at 4° C. Nucleus were visualized using DAPI.
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Immunohistochemical analysis of human breast tissue staining CTNNBL1 with ab76243 at 1/100 dilution.
The human breast sections were performed by LeicaDS9800 (Bond™ Polymer Refine Detection). The sections were using Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then labeling ab76243 at 1/100 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-CTNNBL1 antibody [EP2669Y] (ab76243) at 1/5000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary antibody: incubated for 1h at room temperature.
Secondary antibody: incubated for 1h at 1:20,000 at room temperature (ab97051)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab76243 は 3 報の論文で使用されています。
- Kuhny M et al. Disease-associated CTNNBL1 mutation impairs somatic hypermutation by decreasing nuclear AID. J Clin Invest 130:4411-4422 (2020). PubMed: 32484799
- Pawellek A et al. Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. Elife 6:N/A (2017). PubMed: 28884683
- Pourfarzad F et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . PubMed: 23911284