製品の概要

  • 製品名

    Anti-Ctip2 antibody [25B6] - ChIP Grade
    Ctip2 一次抗体 製品一覧
  • 製品の詳細

    Rat monoclonal [25B6] to Ctip2 - ChIP Grade
  • 由来種

    Rat
  • 特異性

    Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • アプリケーション

    適用あり: ICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Guinea pig, Human, Zebrafish, Cynomolgus monkey
  • 免疫原

    Fusion protein corresponding to Human Ctip2.

  • エピトープ

    Between amino acids 1-150 of CTIP2.
  • ポジティブ・コントロール

    • WB: Nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: Adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
  • 特記事項

    Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18523013
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
IP Use at an assay dependent concentration.
IHC-P 1/500.
IHC-Fr 1/500.
ChIP Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • 組織特異性

    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • 配列類似性

    Contains 6 C2H2-type zinc fingers.
  • 細胞内局在

    Nucleus.
  • Information by UniProt
  • 参照データベース

  • 別名

    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

画像

  • ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.
  • Patterns of staining in the frontal cerebral cortex of pups receiving the mitotic marker EDU on either E12 (A) or E16 (B)

    Note the age-related differences in EDU (red) staining: at E12 EDU is found primarily deep in the cortex (bottom of panel), while at E16 it is found in superficial layers (top). In the E12 tissue (A) substantial numbers of cells expressing the deep marker CTIP2 (blue) co-labelled with EDU (pink) while only a few of the cells labelled with CUX also have the maker (yellow). E16 injections (B) labeled cells in layer 3 expressing the superficial marker CART as wells as more superficial cells. The marker labels all proliferating cells, including glial and perivascular cells. Scale bar = 200μm.

    Ctip2 is labelled with ab18465.

    (From Figure 7 of Brunjes et al)

  • Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

    Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

    Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
  • ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.

    See Abreview

  • Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

    Red is beta tubulin staining.
    Green is ab18465.

  • Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

    Lanes 1-2 : Mouse brain tissue lysate at 1.5 µg
    Lane 3 : Mouse brain tissue lysate at 3 µg

    Secondary
    All lanes : IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 100,110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 minutes

    See Abreview

参考文献

This product has been referenced in:

  • Klingler E  et al. A Translaminar Genetic Logic for the Circuit Identity of Intracortically Projecting Neurons. Curr Biol 29:332-339.e5 (2019). Read more (PubMed: 30639110) »
  • Simmons AJ  et al. Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death. Hum Mol Genet N/A:N/A (2019). Read more (PubMed: 30668728) »
See all 327 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 32 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Marmoset spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 May 31 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (e16.5 Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
e16.5 Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 Apr 26 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (e15 brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
e15 brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human cerebral organoids)
Specification
Human cerebral organoids
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde

Radhika Menon

Verified customer

投稿 Jul 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 10 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain, striatum)
Specification
Brain, striatum

Abcam user community

Verified customer

投稿 May 23 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 04 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 04 2016

1-10 of 32 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録