Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: anti rat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
I am not familiar with the distribution pattern of Ctip2 in adult rat brain but, the immunostaining pattern in hippocampus (not shown) is familiar with that seen in published abreviews.
Submitted image shows strong Ctip2-positivity in a subset of striatum cell nuclei.
Some cell nuclei in the frontal cortex and olfactory bulbs are also positive.
More images are to be seen on the Histonet server.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Apr 04 2018