Anti-CTGF 抗体 (ab6992)

Reviews (27)Q&A (34)References (200)

Key features and details

  • Rabbit polyclonal to CTGF
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CTGF antibody
    CTGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CTGF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Sheep, Human
    交差が予測される動物種: Cow

  • 免疫原

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • ポジティブ･コントロール

    • human hippocampal protein extracts (see Ueberham et al paper).

  • 特記事項

      

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Can be blocked with Mouse CTGF peptide (ab7861).

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.

ターゲット情報

  • 機能

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

  • 組織特異性

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

  • 配列類似性

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

画像

  • Western blot - Anti-CTGF antibody (ab6992)
    Western blot - Anti-CTGF antibody (ab6992)This image is courtesy of an anonymous Abreview
    All lanes : Anti-CTGF antibody (ab6992) at 1/1000 dilution

    Lane 1 : Extracellular matric within skeletal muscle from a mouse with fibrosis in the legs
    Lane 2 : Human skeletal muscle whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor® 488-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

    See Abreview

  • Western blot - Anti-CTGF antibody (ab6992)
    Western blot - Anti-CTGF antibody (ab6992)This image is courtesy of an anonymous Abreview
    Anti-CTGF antibody (ab6992) at 1/5000 dilution (in PBS-T for 9 hours at 4°C) + Mouse brain whole cell lysate at 50 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking Step: 5% Milk for 1 hour at 25°C

    See Abreview

参考文献 (200)

ab6992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6992 は 200 報の論文で使用されています。

  • Wilson DH  et al. Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors. Nat Commun 11:445 (2020). PubMed: 31974352
  • Cao Y  et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
  • Yu Q  et al. Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine-functionalized magnetic iron oxide nanoparticles on LX-2 cells. Mol Med Rep 21:181-190 (2020). PubMed: 31746398
  • Fallah M  et al. Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds. Cell Death Dis 11:201 (2020). PubMed: 32205839
  • Omori H  et al. YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma. Sci Adv 6:eaay3324 (2020). PubMed: 32206709
View all Publications for this product

レビューと Q&A

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1-10 of 61 Abreviews or Q&A

Question

IHC-P with mouse skin no signal tried with a nd withour AR (citrate buffer, microwave) warm vial (was left out for 2 days on loading dock) used same Ab before and worked, different lot Ab: 1/100 - 1/1000 several dilutions, o/n 4 degree block with goat serum 10% + TX-100 secondary: fine

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
30 µg
Treatment
particles
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Pureunhaneul Lee

Verified customer

投稿 Dec 25 2019

Western blot abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
None
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
Permeabilization
No
Specification
Jejunum
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 19 2017

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (iPSC derived nuerons)
Specification
iPSC derived nuerons
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 26 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (saphenous vein)
Specification
saphenous vein
Permeabilization
Yes - tween20
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 19 2014

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (retina)
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 12 2014

Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 03 2014

1-10 of 61 Abreviews or Q&A

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