Anti-CTGF 抗体 (ab6992)

Reviews (27)Q&A (34)References (261)

Key features and details

  • Rabbit polyclonal to CTGF
  • Suitable for: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-CTGF antibody [EPR20728] (ab209780)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-CTGF antibody
    CTGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CTGF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Sheep, Human
    交差が予測される動物種: Cow

  • 免疫原

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • ポジティブ･コントロール

    • human hippocampal protein extracts (see Ueberham et al paper).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6992の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (10)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-FoFr (1)
Use at an assay dependent concentration.
WB (10)
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.
IHC-Fr (2)
Use at an assay dependent concentration.

See Ueberham et al.
IP
Use at an assay dependent concentration. PubMed: 16100286
特記事項
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.
IHC-Fr
Use at an assay dependent concentration.

See Ueberham et al.
IP
Use at an assay dependent concentration. PubMed: 16100286

ターゲット情報

  • 機能

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

  • 組織特異性

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

  • 配列類似性

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

参考文献 (261)

ab6992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6992 は 261 報の論文で使用されています。

  • Zhang Y  et al. miR-212 promotes renal interstitial fibrosis by inhibiting hypoxia-inducible factor 1-a inhibitor. Mol Med Rep 23:N/A (2021). PubMed: 33495813
  • Lyu L  et al. Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress. J Cell Mol Med 25:3136-3148 (2021). PubMed: 33560596
  • Zhu F  et al. Tubular Numb promotes renal interstitial fibrosis via modulating HIF-1a protein stability. Biochim Biophys Acta Mol Basis Dis 1867:166081 (2021). PubMed: 33486098
  • Tokuda K  et al. Development of a novel knee contracture mouse model by immobilization using external fixation. Connect Tissue Res N/A:1-14 (2021). PubMed: 33602048
  • Liu Y  et al. Inhibiting miR-1 attenuates pulmonary arterial hypertension in rats. Mol Med Rep 23:N/A (2021). PubMed: 33604679
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 61 Abreviews or Q&A

Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
30 µg
Treatment
particles
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Pureunhaneul Lee

Verified customer

投稿 Dec 25 2019

Western blot abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
None
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
Permeabilization
No
Specification
Jejunum
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 19 2017

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (iPSC derived nuerons)
Specification
iPSC derived nuerons
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 26 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (saphenous vein)
Specification
saphenous vein
Permeabilization
Yes - tween20
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 19 2014

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (retina)
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 12 2014

Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 03 2014

Question

IHC-P with mouse skin no signal tried with a nd withour AR (citrate buffer, microwave) warm vial (was left out for 2 days on loading dock) used same Ab before and worked, different lot Ab: 1/100 - 1/1000 several dilutions, o/n 4 degree block with goat serum 10% + TX-100 secondary: fine

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

1-10 of 61 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com