Anti-CTCF 抗体 [EPR7313] - BSA and Azide free (ab248214)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7313] to CTCF - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CTCF antibody [EPR7313] - BSA and Azide free
CTCF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7313] to CTCF - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab248214 is the carrier-free version of ab128909.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.58 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7313 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 83 kDa. |
ターゲット情報
-
機能
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. -
組織特異性
Ubiquitous. Absent in primary spermatocytes. -
配列類似性
Belongs to the CTCF zinc-finger protein family.
Contains 11 C2H2-type zinc fingers. -
ドメイン
The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites. -
翻訳後修飾
Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter. -
細胞内局在
Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. - Information by UniProt
-
参照データベース
- Entrez Gene: 10664 Human
- Entrez Gene: 13018 Mouse
- Entrez Gene: 83726 Rat
- Omim: 604167 Human
- SwissProt: P49711 Human
- SwissProt: Q61164 Mouse
- SwissProt: Q9R1D1 Rat
- Unigene: 368367 Human
see all -
別名
- 11 zinc finger protein antibody
- 11 zinc finger transcriptional repressor antibody
- 11-zinc finger protein antibody
see all
画像
-
All lanes : Anti-CTCF antibody [EPR7313] (ab128909) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Caco-2 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDaThis data was developed using ab128909, the same antibody clone in a different buffer formulation.
-
This data was developed using ab128909, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248214 は論文での使用が確認できていません。