Anti-CSRP3 抗体 [EPR12615(B)] (ab172952)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12615(B)] to CSRP3
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CSRP3 antibody [EPR12615(B)]
CSRP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12615(B)] to CSRP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Synthetic peptide within Human CSRP3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P50461 -
ポジティブ・コントロール
- Fetal heart, human skeletal muscle, fetal muscle lysates, human heart tissue, human uterus tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12615(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172952の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. -
組織特異性
Cardiac and slow-twitch skeletal muscles. -
関連疾患
Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
配列類似性
Contains 2 LIM zinc-binding domains. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3. - Information by UniProt
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参照データベース
- Entrez Gene: 540407 Cow
- Entrez Gene: 8048 Human
- Entrez Gene: 13009 Mouse
- Entrez Gene: 117505 Rat
- Omim: 600824 Human
- SwissProt: Q4U0T9 Cow
- SwissProt: P50461 Human
- SwissProt: P50462 Mouse
see all -
別名
- cardiac antibody
- Cardiac LIM protein antibody
- CLP antibody
see all
画像
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All lanes : Anti-CSRP3 antibody [EPR12615(B)] (ab172952) at 1/1000 dilution
Lane 1 : Fetal heart
Lane 2 : Human skeletal muscle
Lane 3 : Fetal muscle
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling CSRP3 with ab172952 at 1/1000 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling CSRP3 with ab172592 at 1/1000 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal lung tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal brain tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1X PBS (negative control) using ab172952, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab172952 は論文での使用が確認できていません。