Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5139] to CSN8 - BSA and Azide free
- Suitable for: ICC, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-CSN8 antibody [EPR5139] - BSA and Azide free
CSN8 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5139] to CSN8 - BSA and Azide free
アプリケーション適用あり: ICC, WB, IHC-P, IPmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human CSN8 aa 150-250 (C terminal). The exact sequence is proprietary.
ab248001 is the carrier-free version of ab124779 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248001 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as COPS8
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab248001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|IP||Use at an assay dependent concentration.|
機能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
配列類似性Belongs to the CSN8 family.
Contains 1 PCI domain.
- Information by UniProt
- COP9 homolog antibody
- COP9 signalosome complex subunit 8 antibody
- COP9 signalosome subunit 8 antibody
All lanes : Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution
Lane 1 : Fetal liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
This data was developed using ab124779, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.