Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6465] to CSN7b - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-CSN7b antibody [EPR6465] - BSA and Azide free
CSN7b 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6465] to CSN7b - BSA and Azide free
アプリケーション適用あり: ICC/IF, Flow Cyt, WB, IPmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human CSN7b aa 200-300. The exact sequence is proprietary.
- WB: HEK293T, HeLa and HepG2 cell lysates.
Ab247979 is the carrier-free version of ab124718. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247979 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.|
|IP||Use at an assay dependent concentration.|
機能Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
配列類似性Belongs to the CSN7/EIF3M family. CSN7 subfamily.
Contains 1 PCI domain.
- Information by UniProt
- COP9 Complex Homolog Subunit 7b antibody
- COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
- COP9 Signalasome Subunit 7b antibody
All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : COPS7B knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab124718).
ab124718 Anti-CSN7b antibody [EPR6465] was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab124718 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.