Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6771] to CSK - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-CSK antibody [EPR6771] - BSA and Azide free
CSK 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6771] to CSK - BSA and Azide free
アプリケーション適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC,ICC/IF or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human CSK aa 50-150. The exact sequence is proprietary.
Ab248095 is the carrier-free version of ab125005. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248095 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 5.10 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
機能Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
組織特異性Expressed in lung and macrophages.
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
翻訳後修飾Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
細胞内局在Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
- Information by UniProt
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
This data was developed using ab125005, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.