Anti-CSK 抗体 (ab744)
Key features and details
- Goat polyclonal to CSK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CSK antibody
CSK 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CSK -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog
-
免疫原
Synthetic peptide corresponding to Human CSK aa 438-450 (C terminal).
Sequence:C-EQLEHIKTHELHL
(Peptide available asab19039) -
ポジティブ･コントロール
- Jurkat cell lysate, Human colon tissue.
-
特記事項
GenBank Accession Number – NP_004374.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
|
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
-
機能
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. -
組織特異性
Expressed in lung and macrophages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. -
細胞内局在
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts. - Information by UniProt
-
参照データベース
- Entrez Gene: 509246 Cow
- Entrez Gene: 487649 Dog
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Entrez Gene: 315707 Rat
- Omim: 124095 Human
- SwissProt: Q0VBZ0 Cow
- SwissProt: P41240 Human
see all -
別名
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
see all
画像
-
Western blot - Anti-CSK antibody (ab744)ab744 staining (0.5µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. -
Western blot - Anti-CSK antibody (ab744)Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
プロトコール
データシートおよび資料
参考文献
ab744 は 2 報の論文で使用されています。
- Yeh WL et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577
