Anti-CSK 抗体 (ab744)

Submit a review Q&A (1)References (2)

製品の概要

  • 製品名
    Anti-CSK antibody
    CSK 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CSK
  • 由来種
    Goat
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human CSK aa 438-450 (C terminal).
    Sequence:

    C-EQLEHIKTHELHL


    (Peptide available as ab19039)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Jurkat cell lysate, Human colon tissue.
  • 特記事項
    GenBank Accession Number – NP_004374.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human CSK peptide (ab19039).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • 組織特異性
    Expressed in lung and macrophages.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • 細胞内局在
    Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

画像

  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa

参考文献

This product has been referenced in:
  • Yeh WL  et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . Read more (PubMed: 23593342) »
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . Read more (PubMed: 18701577) »
See all 2 Publications for this product

ab744 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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Question

Has this antibody ever been tested on any samples other than Jurkat lysate?

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Answer

Thank you for your enquiry. I contacted the originator of this antibody and they said they also did a preliminary Western blot with mouse spleen lysate and saw a band at approximately 45 kDa - but they aren't sure if it's correct. Please let me know if you need additional assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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