リコンビナントRabMAb

Anti-CSF-1-R 抗体 [EPR23529-26] - BSA and Azide free (ab272049)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
  • Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free
    CSF-1-R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates. IHC-P: and Mouse spleen, Mouse liver tissues. ICC/IF: RAW264.7 cell. IP: RAW 264.7 cell.

  • 特記事項

    ab272049 is the carrier-free version of ab254357. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Mouse
IHC-P
Mouse
IP
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.

  • 組織特異性

    Expressed in bone marrow and in differentiated blood mononuclear cells.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C FMS antibody
      • CD 115 antibody
      • CD115 antibody
      • CD115 antigen antibody
      • CFMS antibody
      • Colony stimulating factor 1 receptor antibody
      • Colony stimulating factor I receptor antibody
      • CSF 1 R antibody
      • CSF 1R antibody
      • CSF-1 receptor antibody
      • CSF-1-R antibody
      • CSF1 R antibody
      • CSF1R antibody
      • CSF1R_HUMAN antibody
      • CSFR antibody
      • EC 2.7.10.1 antibody
      • FIM 2 antibody
      • FIM2 antibody
      • FMS antibody
      • FMS proto oncogene antibody
      • FMS protooncogene antibody
      • HDLS antibody
      • M-CSF Receptor antibody
      • M-CSF-R antibody
      • Macrophage colony stimulating factor 1 receptor antibody
      • Macrophage colony stimulating factor I receptor antibody
      • Macrophage colony-stimulating factor 1 receptor antibody
      • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
      • MCSFR antibody
      • Oncogen FMS antibody
      • Proto-oncogene c-Fms antibody
      • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

      Lane 2: ab254357 IP in RAW 264.7 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254357 in RAW 264.7 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 128 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

    • Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
      Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272049 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272049 は論文での使用が確認できていません。

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